Team:Osaka/Notebook

(Difference between revisions)

Revision as of 13:17, 13 October 2010

ID	Part Name	Resistance	Description
R1	<bbpart>BBa_K416003</bbpart>	A,K	yeast secretion tag
R2	<bbpart>BBa_K416004</bbpart>	A	Aga2 yeast cell surface display tag with linker

Miniprep of 026, 028, 030
Restriction digest
- 026, 028 with XbaI, PstI
- 030 with EcoRI, SpeI or EcoRI only
Gel electrophoresis
(RESULTS?)
Transfer to solution culture: 021, 025, 026, 034, 036

'K1' (mentioned on 9/250 -> where did it come from?
9/25 - miniprepped parts (inoculated 9/24) were plasmids from karita sensei? biobrick parts pcr-cloned on 9/23? why where these parts transformed immediately after miniprep?
'CelB, Cel44A' etc vs 'Cel5, Cel8, Cel44' which nomenclature refers to what (received plasmids, pcr product?)

@@ Line 322: / Line 322: @@
 ==Notebook==
+===October 1 (Fri)===
+# Gel electrophoresis (?)
+# Ligations
+#* 006: 004 as upstream, FcenA as downstream, 1-5A as vector
+#* 007: 005 as upstream, FcenA as downstream, 1-5A as vector
+#* 010: 004 as upstream, Fcex as downstream, 1-5A as vector
+#* 011: 005 as upstream, Fcex as downstream, 1-5A as vector
+#* 036: 032 as upstream, 033 as downstream, 1-5A as vector
+#* 037: 004 as upstream, 024 as downstream, 1-1C as vector
+#* 038: 005 as upstream, 024 as downstream, 1-1C as vector
+# Transformation of ligation products
+# PCR of ENO2 promoter, ADH2 promoter, SUC2 signal sequence
+# Parts from NYU team:
+{|
+!ID!!Part Name!!Resistance!!Description
+|-
+|R1||<bbpart>BBa_K416003</bbpart>||A,K||yeast secretion tag
+|-
+|R2||<bbpart>BBa_K416004</bbpart>||A||Aga2 yeast cell surface display tag with linker
+|}
+# Miniprep of 026, 028, 030
+# Restriction digest
+#* 026, 028 with XbaI, PstI
+#* 030 with EcoRI, SpeI or EcoRI only
+# Gel electrophoresis
+# (RESULTS?)
+# Transfer to solution culture: 021, 025, 026, 034, 036
 ===TO CHECK/CONFIRM===
 * 'K1' (mentioned on 9/250 -> where did it come from?