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<table width=70%><tr><td><div id="bodybaby">the idea</div></td>
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<table width=70%><tr><td><div id="bodybaby">biobrick construction</div></td>
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<b>Antarctic Phosphatase</b><br>
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To prevent recircularization of digested plasmids, we used NEB's Antarctic Phosphatase according to protocol.<br>
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<b>Gel Electrophoresis</b><br>
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To view lengths of DNA, Orange G 6x was added 1:3 to 300ng miniprepped DNA and run against 5uL of Hyperladder I in the lanes of 1% UltraPure agarose gels in TAE buffer at 120V for 45 minutes.<br>
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<b>DNA Gel Extraction</b><br>
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To extract the DNA from the agarose gel, the Zymo Gel Extraction kit was used according to protocol.</td>
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Revision as of 15:53, 10 October 2010

MIT iGEM 2010


Bacterial Construction Protocol
Bacterial Experimental Protocol
Phage western blot
Mammalian Cell Lines
Microfluidic stress

Materials

biobrick construction

Antarctic Phosphatase
To prevent recircularization of digested plasmids, we used NEB's Antarctic Phosphatase according to protocol.
Gel Electrophoresis
To view lengths of DNA, Orange G 6x was added 1:3 to 300ng miniprepped DNA and run against 5uL of Hyperladder I in the lanes of 1% UltraPure agarose gels in TAE buffer at 120V for 45 minutes.
DNA Gel Extraction
To extract the DNA from the agarose gel, the Zymo Gel Extraction kit was used according to protocol.

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