Team:Harvard/flavor/parts
From 2010.igem.org
(Difference between revisions)
Line 39: | Line 39: | ||
<tr><td>NOSterm_BB_R</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | <tr><td>NOSterm_BB_R</td><td>smGFP-NheI reverse primer for Reporter Series 1 insert</td><td></td></tr> | ||
<tr><td>Stop_NOSterm_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr> | <tr><td>Stop_NOSterm_BB_F</td><td>Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert</td><td></td></tr> | ||
- | <tr><td>pENTCUP2_BB_F</td><td> | + | <tr><td>pENTCUP2_BB_F</td><td>Plant specific promoter forward primer</td><td></td></tr> |
- | <tr><td>pENTCUP2_BB_R</td><td> | + | <tr><td>pENTCUP2_BB_R</td><td>Plant specific promoter reverse primer</td><td></td></tr> |
Revision as of 15:39, 10 October 2010
![](https://static.igem.org/mediawiki/2010/f/f3/Harvard2010flavorbanner.png)
parts and primers
Primers
Name | Description | Sequence |
J45004_F | HindIII-pENTCUP2 forward primer for Expression Series insert | |
J45004_R | pENTCUP2-biobrick multiple cloning site-NheI reverse primer for Expression Series insert | |
J45017_F | HindIII-biobrick multiple cloning site-gusA-forward primer for Reporter Series 1 insert | |
J45017_R | gusA-NheI reverse primer for Reporter Series 1 insert | |
NOSterm_BB_F | HindIII-biobrick multiple cloning site-smGFP-forward primer for Reporter Series 1 insert | |
NOSterm_BB_R | smGFP-NheI reverse primer for Reporter Series 1 insert | |
Stop_NOSterm_BB_F | Biobrick multiple cloning site for annealing with VP10 to create Open Series Insert | |
pENTCUP2_BB_F | Plant specific promoter forward primer | |
pENTCUP2_BB_R | Plant specific promoter reverse primer |