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| - | <table width=70%><tr><td id="bodybaby">the bacterial uv toggle</td> | + | <table width=70%><tr><td><div id="bodybaby">the bacterial uv toggle</div></td> |
<tr><td> <br>In the beginning, there was a UV Toggle (<a href="http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html">Collins, 2000</a>). <br> The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. The team planned for the toggle to control fluorescence and phage polymerization. At first there was a pattern of fluorescence induced by UV exposure -- the first image.<br> | <tr><td> <br>In the beginning, there was a UV Toggle (<a href="http://www.nature.com/nature/journal/v403/n6767/abs/403339a0.html">Collins, 2000</a>). <br> The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. The team planned for the toggle to control fluorescence and phage polymerization. At first there was a pattern of fluorescence induced by UV exposure -- the first image.<br> | ||
<a href="https://static.igem.org/mediawiki/2010/8/80/First.png" class="thickbox" title="The first patterned image created by exposing masked cells to UV light. The cells were made by co-transforming the Collins toggle plasmid pTSMa with our composite biobrick K415013."><img style="margin: 0px 20%;" height=200px src="https://static.igem.org/mediawiki/2010/8/80/First.png"></a><br> <br> | <a href="https://static.igem.org/mediawiki/2010/8/80/First.png" class="thickbox" title="The first patterned image created by exposing masked cells to UV light. The cells were made by co-transforming the Collins toggle plasmid pTSMa with our composite biobrick K415013."><img style="margin: 0px 20%;" height=200px src="https://static.igem.org/mediawiki/2010/8/80/First.png"></a><br> <br> | ||
Revision as of 21:42, 9 October 2010
iGEM 2010
Undergraduates Instructors Advisors Fun Sponsors
Overview Toggle Phage Mammalian Summary Acknowledgements
Materials & Methods Biosafety Journal Club
the bacterial uv toggle
In the beginning, there was a UV Toggle (Collins, 2000).
The 2010 MIT iGEM team saw that it was good, and decided to implement the Collins toggle in E.coli to create cells with bistable phenotypes. The team planned for the toggle to control fluorescence and phage polymerization. At first there was a pattern of fluorescence induced by UV exposure -- the first image.
Then the team said let's make the signal amplify itself, and make a movie to see its propogation. And it was so.
Here we see cells controlled by the Low Power Toggle. The cells fluoresce red with UV induction, but at higher UV levels cell death can be seen in the green field.
