Team:Kyoto/Parts

From 2010.igem.org

(Difference between revisions)
(BioBrick)
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==BioBrick==
==BioBrick==
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===Parts===
{| class="parts sortable"
{| class="parts sortable"
!Name||Description||Well<sup>*1</sup>||Plasmid||Resistance||Insert Length||Vector Length
!Name||Description||Well<sup>*1</sup>||Plasmid||Resistance||Insert Length||Vector Length
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|<partinfo>B0015</partinfo>||Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)||1-23-L||<partinfo>pSB1AK3</partinfo>||Ampicillin, Kanamycin||129||3189
|<partinfo>B0015</partinfo>||Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)||1-23-L||<partinfo>pSB1AK3</partinfo>||Ampicillin, Kanamycin||129||3189
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|<partinfo>pSB4K5</partinfo>||Low copy BioBrick standard vector||1-5-G||<partinfo>pSB4K5</partinfo>||Kanamycin||1069||3419
 
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|<partinfo>pSB1C3</partinfo>||High copy BioBrick assembly plasmid||||<partinfo>pSB1C3</partinfo>||Chloramphenicol||||2072
 
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|<partinfo>E0840</partinfo>||GFP generator||1-12-O||<partinfo>pSB1A2</partinfo>||Ampicillin||878||2079
|<partinfo>E0840</partinfo>||GFP generator||1-12-O||<partinfo>pSB1A2</partinfo>||Ampicillin||878||2079
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|<partinfo>pSB3K3</partinfo>||RFP Coding Device||1-5-E||<partinfo>pSB3K3</partinfo>||Kanamycin||1069||2750
|<partinfo>pSB3K3</partinfo>||RFP Coding Device||1-5-E||<partinfo>pSB3K3</partinfo>||Kanamycin||1069||2750
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|-
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|<partinfo>pSB4K5</partinfo>||Low copy BioBrick standard vector||1-5-G||<partinfo>pSB4K5</partinfo>||Kanamycin||1069||3419
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|-
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|<partinfo>pSB1C3</partinfo>||High copy BioBrick assembly plasmid||||<partinfo>pSB1C3</partinfo>||Chloramphenicol||||2072
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|-
|}
|}
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* *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.
* *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.
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===Primers===
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{| class="parts sortable"
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!Name||Description||Sequence
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|-
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|<partinfo>G00100</partinfo>||Forward primer for sequencing/amplifying BioBrick parts (VF2)||tgccacctgacgtctaagaa
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|-
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|<partinfo>G00101</partinfo>||Reverse primer for sequencing/amplifying BioBrick parts (VR)||attaccgcctttgagtgagc
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|}
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----
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Revision as of 16:53, 9 October 2010

Contents


Original

<groupparts>iGEM010 Kyoto</groupparts>

BioBrick

Parts

NameDescriptionWell*1PlasmidResistanceInsert LengthVector Length
<partinfo>J23100</partinfo>Constitutive promoter family member1-18-C<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23105</partinfo>Constitutive promoter family member1-18-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>J23116</partinfo>Constitutive promoter family member1-20-M<partinfo>J61002</partinfo>Ampicillin352948
<partinfo>R0011</partinfo>Promoter (lacI regulated, lambda pL hybrid)1-6-G<partinfo>pSB1A2</partinfo>Ampicillin552079
<partinfo>B0015</partinfo>Double terminator (<partinfo>B0010</partinfo>-<partinfo>B0012</partinfo>)1-23-L<partinfo>pSB1AK3</partinfo>Ampicillin, Kanamycin1293189
<partinfo>E0840</partinfo>GFP generator1-12-O<partinfo>pSB1A2</partinfo>Ampicillin8782079
<partinfo>E0240</partinfo>GFP generator1-12-M<partinfo>pSB1A2</partinfo>Ampicillin8762079
<partinfo>I20260</partinfo>Measurement Kit Test of <partinfo>J23101</partinfo>2-17-F<partinfo>pSB3K3</partinfo>Kanamycin9192750
<partinfo>J06702</partinfo>mCherry, bacterial with RBS and forward terminator2-8-E<partinfo>pSB1A2</partinfo>Ampicillin8692079
<partinfo>pSB3K3</partinfo>RFP Coding Device1-5-E<partinfo>pSB3K3</partinfo>Kanamycin10692750
<partinfo>pSB4K5</partinfo>Low copy BioBrick standard vector1-5-G<partinfo>pSB4K5</partinfo>Kanamycin10693419
<partinfo>pSB1C3</partinfo>High copy BioBrick assembly plasmid<partinfo>pSB1C3</partinfo>Chloramphenicol2072
  • *1 "1-18-C" means well 18C in [http://partsregistry.org/Help:Spring_2010_DNA_distribution Spring 2010 DNA Distribution Kit] Plate 1.

Primers

NameDescriptionSequence
<partinfo>G00100</partinfo>Forward primer for sequencing/amplifying BioBrick parts (VF2)tgccacctgacgtctaagaa
<partinfo>G00101</partinfo>Reverse primer for sequencing/amplifying BioBrick parts (VR)attaccgcctttgagtgagc