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Team:TU Delft/Modeling/MFA/NO3
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In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced. | In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced. | ||
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In this scenario the oxidative phosphorylation was disabled and a replacement was implemented; | In this scenario the oxidative phosphorylation was disabled and a replacement was implemented; | ||
| - | NO3- + NADH + 8 H+ -> NO2- + NAD+ + H+ + | + | NO3- + NADH + 8 H+ -> NO2- + NAD+ + H+ + 4 Hex+ + H2O |
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| - | The goal of implementing this pathway is to | + | This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP. Less protons are exported per mol of NADH, so the ATP/NADH ratio will drop compared to oxygen. The goal of implementing this pathway however, is to see how much the oxygen requirement of ''E. coli'' can be reduced. |
[[Team:TU_Delft/Modeling/MFA|go back]] | [[Team:TU_Delft/Modeling/MFA|go back]] | ||
Latest revision as of 13:20, 7 October 2010
NO3 as electron acceptor
In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced.
In this scenario the oxidative phosphorylation was disabled and a replacement was implemented;
NO3- + NADH + 8 H+ -> NO2- + NAD+ + H+ + 4 Hex+ + H2O
This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP. Less protons are exported per mol of NADH, so the ATP/NADH ratio will drop compared to oxygen. The goal of implementing this pathway however, is to see how much the oxygen requirement of E. coli can be reduced.
