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O/N Culture
From 2010.igem.org
(Difference between revisions)
(New page: '''REMEMBER TO BE STERILE''' *Take 5mL LB and put in a round bottom tube *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube '''MAKE SURE YOU ADD THE RIGHT ...) |
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*Take 5mL LB and put in a round bottom tube | *Take 5mL LB and put in a round bottom tube | ||
| - | *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube '''MAKE SURE YOU ADD THE RIGHT ONE!''' | + | *If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube |
| + | *'''MAKE SURE YOU ADD THE RIGHT ONE!''' | ||
*Take a tip on a p200 and lightly touch a colony | *Take a tip on a p200 and lightly touch a colony | ||
*Eject the tip into the culture and put the cap on | *Eject the tip into the culture and put the cap on | ||
*For a miniprep, grow O/N for 12-16 hours NOT LONGER | *For a miniprep, grow O/N for 12-16 hours NOT LONGER | ||
Revision as of 17:36, 14 June 2010
REMEMBER TO BE STERILE
- Take 5mL LB and put in a round bottom tube
- If your antibiotic is 1000x (which it should be) add 5uL antibiotic to your tube
*MAKE SURE YOU ADD THE RIGHT ONE!
- Take a tip on a p200 and lightly touch a colony
- Eject the tip into the culture and put the cap on
- For a miniprep, grow O/N for 12-16 hours NOT LONGER
