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Team:HokkaidoU Japan/Notebook/September10
From 2010.igem.org
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=3 piece ligation, continued= | =3 piece ligation, continued= | ||
| - | * Yesterday after purification by gel extraction electrophoresis showed very weak bands | + | * Yesterday after purification by [[Team:HokkaidoU_Japan/Protocols|gel extraction]] electrophoresis showed very weak bands |
* This time increased PCR cycles to 40 | * This time increased PCR cycles to 40 | ||
* Everything else was same as last time | * Everything else was same as last time | ||
Latest revision as of 09:24, 2 October 2010
since 13 Jul 2010
since 1 Aug 2010
3 piece ligation, continued
- Yesterday after purification by gel extraction electrophoresis showed very weak bands
- This time increased PCR cycles to 40
- Everything else was same as last time
- But again it was a failure
- Thought that template went bad，so used PCR Product as template
- Extension was 90 sec, did 30 cycles
