Team:HokkaidoU Japan/Notebook/September10

From 2010.igem.org

(Difference between revisions)
(→3 piece ligation, continued)
 
(3 intermediate revisions not shown)
Line 1: Line 1:
-
{{Template:HokkaidoU_Japan}}
+
{{Template:HokkaidoU_Japan}}<div class="linkbar"><div class="prev">[[Team:HokkaidoU_Japan/Notebook/September9|September 9]]</div>[[Team:HokkaidoU_Japan/Notebook|Notebook]]<div class="next">[[Team:HokkaidoU_Japan/Notebook/September13|September 13]]</div></div>
-
=3 piece ligation つづき=
+
 
-
* 前日にPCRしたHSPとGFP全量を電気泳動にかけたが，くっきりしたバンドがみられなかった
+
=3 piece ligation, continued=
-
* PCRでDNAが十分に増幅されなかったものと考え，cycle数を40回に増やしてもう一度PCRからやった
+
 
-
* これを同様に電気泳動したが，やはり増幅がうまくいかなかった
+
* Yesterday after purification by [[Team:HokkaidoU_Japan/Protocols|gel extraction]] electrophoresis showed very weak bands
-
* Templateが悪いと考え，PCRで増やしたこれらのパーツを鋳型に，Extension 90 sec, cycle数30回でもう一度PCRを行った
+
* This time increased PCR cycles to 40
 +
* Everything else was same as last time
 +
* But again it was a failure
 +
* Thought that template went bad，so used PCR Product as template
 +
* Extension was 90 sec, did 30 cycles

Latest revision as of 09:24, 2 October 2010

Notebook

3 piece ligation, continued

  • Yesterday after purification by gel extraction electrophoresis showed very weak bands
  • This time increased PCR cycles to 40
  • Everything else was same as last time
  • But again it was a failure
  • Thought that template went bad，so used PCR Product as template
  • Extension was 90 sec, did 30 cycles
Retrieved from "http://2010.igem.org/Team:HokkaidoU_Japan/Notebook/September10"