Team:Edinburgh/BioBricks

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Revision as of 15:10, 28 September 2010

BioBricks: Genomic BRIDGEs

For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).

Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.

In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!

Name	Type	Link	Description
cat	Protein Domain	BBa_K322210	chloramphenicol acetyltransferase (chloramphenicol resistance gene)
sacB	Coding	BBa_K322921	Bacillus subtilis levansucrase, lethal to E. coli in presence of sucrose

BioBricks: Bacterial BRIDGEs

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Name	Type	Link	Description
luxAB	Coding	BBa_K322139	Bacterial luciferase producing blue light.
Codon optimised bright firefly luciferase	Coding	BBa_K322451	Photinus pyralis luciferase mutated for increased bioluminescence.

@@ Line 137: / Line 137: @@
     <th width="100px"><u>Link</u></th>
     <th><u>Description</u></th>
+ </tr>
   <tr>
    <td><b>cat</b></td>
@@ Line 173: / Line 174: @@
     <th width="100px"><u>Link</u></th>
     <th><u>Description</u></th>
+ </tr>
+ <tr>
+  <td align="left"><b>luxAB</b></td>
+  <td>Coding</td>
+  <td><a href="http://partsregistry.org/Part:BBa_K322139">BBa_K322139</a></td>
+  <td>Bacterial luciferase producing blue light.</td>
+ </tr>
   <tr>
    <td align="left"><b>Codon optimised bright firefly luciferase</b></td>