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Team:Edinburgh/BioBricks
From 2010.igem.org
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<li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a> | <li><a href="https://2010.igem.org/Team:Edinburgh/Human" class="dir">human BRIDGEs</a> | ||
<ul> | <ul> | ||
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Human">human aspects</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Aspects">human aspects</a></li> |
| + | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Epic">the epic</a></li> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">results</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Results#Human">results</a></li> | ||
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Human">future work</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/Future">future work</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Human">references</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Human/References">references</a></li> |
</ul> | </ul> | ||
</li> | </li> | ||
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<ul> | <ul> | ||
<li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">collaboration</a></li> | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">collaboration</a></li> | ||
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">BRIDGE</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/BRIDGE">BRIDGE</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">red light</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Red_light_producer">red light</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">red sensor</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Red_light_sensor">red sensor</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">blue light</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Blue_light_producer">blue light</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook">blue sensor</a></li> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Blue_light_sensor">blue sensor</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook"> | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Modelling">modelling</a></li> |
| - | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook | + | <li><a href="https://2010.igem.org/Team:Edinburgh/Notebook/Safety">safety</a></li> |
| - | + | ||
<li><a href="http://www.openwetware.org/wiki/French_Lab">protocols</a></li> | <li><a href="http://www.openwetware.org/wiki/French_Lab">protocols</a></li> | ||
</ul> | </ul> | ||
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<p>In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed <i>E. coli</i> no longer smells!</p> | <p>In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed <i>E. coli</i> no longer smells!</p> | ||
| + | |||
| + | <br> | ||
| + | |||
| + | <table border="0" cellspacing="10" cellpadding="5" style="background:transparent; border-color:transparent;"> | ||
| + | <tr> | ||
| + | <th width="100px"><u>Name</u></th> | ||
| + | <th width="100px"><u>Type</u></th> | ||
| + | <th width="100px"><u>Link</u></th> | ||
| + | <th><u>Description</u></th> | ||
| + | <tr> | ||
| + | <td><b>cat</b></td> | ||
| + | <td>Protein Domain</td> | ||
| + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K322210">BBa_K322210</a></td> | ||
| + | <td>chloramphenicol acetyltransferase (chloramphenicol resistance gene)</td> | ||
| + | </tr> | ||
| + | <tr> | ||
| + | <td><b>sacB</b></td> | ||
| + | <td>Coding</td> | ||
| + | <td><a href="http://partsregistry.org/wiki/index.php?title=Part:BBa_K322921">BBa_K322921</a></td> | ||
| + | <td>Bacillus subtilis levansucrase, lethal to <i>E. coli</i> in presence of sucrose</td> | ||
| + | </tr> | ||
| + | </table> | ||
<br> | <br> | ||
Revision as of 10:57, 28 September 2010
BioBricks: Genomic BRIDGEs
For this part of the project we will have one main biobrick, which will be the construct required for the two-step markerless insertion, encompassing cat (chloramphenicol resistance) and sacB (prevents growth on sucrose).
Chloramphenicol resistance has already been well characterised in the registry, so our characterisation has mainly focused on sacB.
In addition we will probably be submitting the construct with the up- and down- stream sequences of useful genes which can be removed, e.g. tnaA, which produces indole - when removed E. coli no longer smells!
| Name | Type | Link | Description |
|---|---|---|---|
| cat | Protein Domain | BBa_K322210 | chloramphenicol acetyltransferase (chloramphenicol resistance gene) |
| sacB | Coding | BBa_K322921 | Bacillus subtilis levansucrase, lethal to E. coli in presence of sucrose |
BioBricks: Bacterial BRIDGEs
Biobrick #1
A brighter luciferase: we had synthesised a version of the Photinus pyralis (Ppy) luciferase with 3 specific amino acid mutations: Ile423Leu Asp436Gly and Leu530Arg. These mutations increase the light intensity of the luciferase by a factor of 12.5.
Currently, this part is only present in sequence form. Physical DNA will be submitted shortly.
Reference: Fujii H, Noda K, Asami Y, Kuroda A, Sakata M, Tokida A (2007) Increase in bioluminescence intensity of firefly luciferase using genetic modification. Analytical Biochemistry 366: 131-136
