Team:Lethbridge/Notebook/Lab Work/June
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JustinVigar (Talk | contribs) (→June 2/2010) |
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<tr><td>Orange Buffer (10x)</td><td>2</td></tr> | <tr><td>Orange Buffer (10x)</td><td>2</td></tr> | ||
<tr><td>pDNA (rbs-xylE)</td><td>2</td></tr> | <tr><td>pDNA (rbs-xylE)</td><td>2</td></tr> | ||
- | </table> | + | </table> <br> |
+ | |||
+ | I started ran the reaction for 80 minutes at 37<sup>o</sup>C.<br> | ||
===June 3/2010=== | ===June 3/2010=== | ||
<b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br> | <b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br> |
Revision as of 23:28, 10 June 2010
Contents |
June 2010
June 1/2010
JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in working plasmids box.
June 2/2010
(In Lab: JV)
Objective: Isolate plasmid DNA (BBa_J33204) from DH5α cells and confirm results.
Method: "Mini-prep" the plasmid DNA using boiling lysis miniprep. Then restrict the DNA once and run on a 1% agarose gel (TAE).
Restriction Reaction
Ingredient | Volume(µL) |
MilliQ H20 Water | 15.75 |
Orange Buffer (10x) | 2 |
pDNA (rbs-xylE) | 2 |
EcoRI | 0.25 |
Unrestricted Control
Ingredient | Volume(µL) |
MilliQ H20 Water | 16 |
Orange Buffer (10x) | 2 |
pDNA (rbs-xylE) | 2 |
I started ran the reaction for 80 minutes at 37oC.