Team:Lethbridge/Notebook/Lab Work/June

From 2010.igem.org

(Difference between revisions)
(June 2/2010)
(June 2/2010)
Line 49: Line 49:
<b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5&alpha; cells and confirm results.<br>
<b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5&alpha; cells and confirm results.<br>
-
<b>Method:</b> "Mini-prep" the plasmid DNA using
+
<b>Method:</b> "Mini-prep" the plasmid DNA using [[Team:Lethbridge/Notebook/Protocols|boiling lysis miniprep]]. Then restrict the DNA once and run on a 1% agarose gel (TAE). <br>
===June 3/2010===
===June 3/2010===
<b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br>
<b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br>

Revision as of 22:53, 10 June 2010

UofLteamlogo.jpg UofLLabWork.JPG UofLteamlogo.jpg

[http://2010.igem.org/wiki/index.php?title=Team:Lethbridge&Home UofLhomebutton.jpg]

[http://2010.igem.org/Team:Lethbridge/Team UofLteambutton.jpg]

[http://2010.igem.org/Team:Lethbridge/Project UofLprojectbutton.jpg]

[http://2010.igem.org/Team:Lethbridge/Parts UofLpartsbutton.jpg]

[http://2010.igem.org/Team:Lethbridge/Modeling UofLmodelingbutton.jpg]

[http://2010.igem.org/Team:Lethbridge/Notebook UofLnotebookbutton.jpg]

Contents

June 2010

June 1/2010

JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in working plasmids box.


June 2/2010

(In Lab: JV)

Objective: Isolate plasmid DNA (BBa_J33204) from DH5α cells and confirm results.

Method: "Mini-prep" the plasmid DNA using boiling lysis miniprep. Then restrict the DNA once and run on a 1% agarose gel (TAE).

June 3/2010

Objective: Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.