Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06

From 2010.igem.org

(Difference between revisions)
(→4:Ligation)
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=2010/09/06=
 
===1:PCR===
===1:PCR===
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<Member>
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<Member><br />
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Hitomi,nito
Hitomi,nito
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<Sample>
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<Sample><br />
:*1(BBa_K208017)
:*1(BBa_K208017)
:*2(BBa_JM109)
:*2(BBa_JM109)
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<Protocol>
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/ Protocol 2 ]
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See Protocol2
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(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)
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Tubes(Temperature in annealing)
Tubes(Temperature in annealing)
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:*5(BBa_K208017) 70.0,70.5,71.0℃
:*5(BBa_K208017) 70.0,70.5,71.0℃
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===2:Purification of PCR===
 
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<Member>
 
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===2:DNA extraction===
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<Member><br />
Hitomi
Hitomi
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<Protocol>
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<Protocol><br />
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_extraction/ Protocol 4 ]
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See Protocol
 
===3:Electrophoresis===
===3:Electrophoresis===
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<Samlpe>
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<Samlpe><br />
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PCR products.
PCR products.
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:*1
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*1
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:*2
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*2
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:*5
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*5
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:*7
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*7
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:*8
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*8
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<Protocol><br />
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<Protocol>
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/ Protocol 8 ]
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See Protocol8
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(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)
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===4:Ligation===
===4:Ligation===
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<Membber><br />
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<Membber>
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-
 
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nito
nito
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<Sample>
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<Sample><br />
-
 
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*7
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:*7
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*8
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:*8
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<Protocol>
<Protocol>
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]
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Revision as of 06:47, 28 September 2010

Contents

1:PCR

<Member>
Hitomi,nito


<Sample>

  • 1(BBa_K208017)
  • 2(BBa_JM109)
  • 5(BBa_K208017)


<Protocol>
See Protocol 2

Tubes(Temperature in annealing)

  • 1(BBa_K208017) 71.5,72.0,72.5℃
  • 2(BBa_JM109) 71.0,71.5,72.0℃
  • 5(BBa_K208017) 70.0,70.5,71.0℃


2:DNA extraction

<Member>
Hitomi


<Protocol>
See Protocol 4


3:Electrophoresis

<Samlpe>
PCR products.

  • 1
  • 2
  • 5
  • 7
  • 8

<Protocol>
See Protocol 8


<Result>

File:Matsuura 2010-09-06 16hr 49min.tif

4:Ligation

<Membber>
nito


<Sample>

  • 7
  • 8


<Protocol> See Protocol 3

Retrieved from "http://2010.igem.org/Team:Tokyo_Metropolitan/Notebook/Pattern/2010/09/06"