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Team:HokkaidoU Japan/Notebook/September13
From 2010.igem.org
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(→Electrophoresed after gel extraction) |
(→GFP(1-12O)ゲル抽精製済の濃度測定) |
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| - | =GFP(1-12O) | + | =Gel purification of GFP(1-12O) and concentration check= |
| + | |||
[[Image:HokkaidoU Japan 20100913c.jpg|200px|right|thumb|]] | [[Image:HokkaidoU Japan 20100913c.jpg|200px|right|thumb|]] | ||
| - | * TSUDA I 2 uL, DNA 0.5 uL | + | |
| - | + | * [https://2010.igem.org/Image:HokkaidoU_Pictures_DNA_Marker.png TSUDA I] 2 uL, DNA 0.5 uL | |
| - | * 878 + 220 | + | →Estimated concentration to be 120 ng/uL |
| + | * Part length is 878 + 220 bp = 947 bp | ||
| + | * That slightly above mark so OK． | ||
Revision as of 06:26, 28 September 2010
since 13 Jul 2010
since 1 Aug 2010
araC promoter purification
Compared to marker band was a little lower than it should. Thinking that this was due to too big an amount, gel extracted anyway.
- Used TSUDA marker
- Part length is 1259 bp
Electrophoresed after gel extraction
- Electrophoresed TSUDA ITSUDA I 2 uL and 0.5 uL of purified solutuion
→Estimated concentration to be 54 ng/uL
- This time band location is good
- Accidentally excised a part of other band resulting small contamination
Gel purification of GFP(1-12O) and concentration check
- TSUDA I 2 uL, DNA 0.5 uL
→Estimated concentration to be 120 ng/uL
- Part length is 878 + 220 bp = 947 bp
- That slightly above mark so OK．
