Team:Newcastle/Filamentous Cells
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'''Filamentous Cells''' | '''Filamentous Cells''' | ||
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|YneA expression via the inactivation of ''dinR'' by Rec A is important. | |YneA expression via the inactivation of ''dinR'' by Rec A is important. | ||
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+ | |'''Sequence of YneA:''' http://www.ncbi.nlm.nih.gov/nuccore/NC_000964.3?from=1918391&to=1918738&report=graph&content=5 | ||
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|} | |} | ||
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'''Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.''' | '''Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.''' | ||
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+ | ===MinCDJ=== | ||
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+ | |In ''B.subtilis'' cell division occurs precisely mid cell via the formation of the FtsZ ring (tubulin homologue), through Noc (Nucleoid occlusion: prevents division over nucleoids) and the min system (well described in E.coli), which prevents division taking place at the ends of the cell (poles). | ||
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+ | |Main function of Min system to prevent mini cell formation and ensuring only one cell division occurs per cell cycle. | ||
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+ | |Min’s role is not just in the inhibition of FtsZ. FtsZ recruits other components causing synthesis of the new wall and cell invagination. | ||
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+ | |[[Image:Min.png]] | ||
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+ | |Cell division is regulated spatially and temporally. | ||
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+ | |Min C inhibits FtsZ ring formation; Min C interacts with Min D via its C-terminal domain. Min C inhibits lateral interaction between the filaments. | ||
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+ | | Min D is a membrane associated ATPase. (Min E ensures high concentrations of MinCD at the poles in ''E.coli'', no Min E homologue in ''B.subtilis!'' Instead DivIVA acts as a topological factor). | ||
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+ | |Min C in ''B.subtilis'' is responsible for localisation (shown using GFP) showed a primary site of localisation at the site of active division challenging the original model for the role of Min. | ||
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+ | |Min J was discovered linking Min D to DivIVA and therefore necessary for localisation. | ||
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+ | |Over expression of Min D in the absence of Min J causes lethal filamentation. | ||
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+ | |[[Image:TableMin.png]] | ||
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+ | |'''van Baarle, S., & Bramkamp, M. (2010). The MinCDJ system in Bacillus subtilis prevents minicell formation by promoting divisome disassembly. PloS one, 5(3), e9850. doi: 10.1371/journal.pone.0009850.''' | ||
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+ | |'''Bramkamp, M. & van Baarle, S., 2009. Division site selection in rod-shaped bacteria. Current opinion in microbiology, 12(6), 683-8. Available at: http://www.ncbi.nlm.nih.gov/pubmed/19884039.''' |
Revision as of 11:05, 10 June 2010
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Filamentous Cells
YneA
Kawai, Y., Moriya, S., & Ogasawara, N. (2003). Identification of a protein, YneA, responsible for cell division suppression during the SOS response in Bacillus subtilis. Molecular microbiology, 47(4), 1113-22. Retrieved from http://www.ncbi.nlm.nih.gov/pubmed/12581363.