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Team:TU Delft/16 September 2010 content
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(New page: ==Emulsifier== Yesterdays cultures grown over night. Since Top10 cells activate Lac promoters by themselves, I did a quick and dirty experiment on those cultures. 1 ml culture was spin do...) |
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Revision as of 12:03, 24 September 2010
Emulsifier
Yesterdays cultures grown over night. Since Top10 cells activate Lac promoters by themselves, I did a quick and dirty experiment on those cultures.
1 ml culture was spin down for 2 min at max speed.
The supernatant was used in the emulsification assay.
| # | Description | OD600 | absorption (493 nm) | absorption (493 nm) with Sudan II |
| 1 | Top 10 (control) | 2.177 | 2.302 | |
| 2 | Top 10 with 206 | 1.703 | 1.862 | |
| 3 | Sup Top 10 (control) | 0.013 | 0.341 | |
| 4 | Sup Top 10 with 206 | 0.015 | 0.349 |
So the conclusion is that there is no difference between the control and the transformed cultures. So we just have to wait until we have the K12 strain.
