Team:LMU-Munich/Notebook

Notebook

You should make use of the calendar feature on the wiki and start a lab notebook. This may be looked at by the judges to see how your work progressed throughout the summer. It is a very useful organizational tool as well.

Protocols

• 1 competent cells
• 2 highly competent cells
• 3 Transformation
• 4 Plasmid extraction from cells
• 5 Restriction digestion
• 6 SOB medium
• 7 Measurement of competence
• 8 SOC medium
• 9 Handling primers
• 10 PCR with Pfu
• 11 Agarose gel electrophoresis
• 12 Gel extraction or PCR Clean up (Promega kit)
• 13 3A Method for Biobrick assembly
• 14 QIAEX II gel extraction
• 15 PCR with Phusion
• 16 PCR with DreamTaq
• 17 Special thermal cycler programs
• 18 competent cells2
• 19 Transformation SSS
• 20 PCR with Phusion Hot Start
• 21 PCR with Taq Mastermix
• 22 Ligation
• 23 LB Medium