From 2010.igem.org
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Handling Primers
source: Alexander Buschle
Handling of primers received:
- Short spin the primers
- Add autoclaved, sterile, distilled water to primers (take volume from accompanying sheet of paper, column: Vol. for 100pmol/µl) and mix gently (don’t vortex).
- Take new tubes and fill in 90µl autoclaved, sterile, distilled water and add 10µl primer stock solution -> 1:10 diluted
- Freeze primer stock solution and use diluted solution
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