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| - | __NOTOC__
| + | {{Template:Uppsala}}<!--Do not remove the first and last lines in this page!--> |
| - | <html>
| + | |
| - | | + | |
| - | | + | |
| - | <style>
| + | |
| - | h1.firstHeading { display: none; }
| + | |
| - | | + | |
| - | p {text-align: justify;}
| + | |
| - | | + | |
| - | a:link { color: #00b0e6; text-decoration: none}
| + | |
| - | a:visited { color:#00b0e6; text-decoration: none}
| + | |
| - | a:hover { color:#f29400; text-decoration: none}
| + | |
| - | a:active { color:#f29400; text-decoration: none}
| + | |
| - | | + | |
| - | #bodyContent { padding: 10px auto; width: 910px; margin: auto; clear: none; }
| + | |
| - | | + | |
| - | table#team_members { text-align: justify; border: 0; }
| + | |
| - | table#team_members h2, table#team_members h3 { clear: both; }
| + | |
| - | | + | |
| - | | + | |
| - | /*-----------------------------------------------------------------------------------------------*/
| + | |
| - | div.MenuBar ul li ul.DropDownMenu {
| + | |
| - | display: none; /* Hides all drop-down menus. */
| + | |
| - | | + | |
| - | }
| + | |
| - | /*
| + | |
| - | li:hover works in IE7 and FF2.
| + | |
| - | a:hover works in IE6 and FF2.
| + | |
| - | a:hover breaks li:hover in FF2.
| + | |
| - | */
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a ul.SideMenu {
| + | |
| - | display: none; /* Hides all side menus. */
| + | |
| - | }
| + | |
| - | /*------------------------------------------------------------------------------------- Menu Bar */
| + | |
| - | div.MenuBar {
| + | |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | height: 30px;
| + | |
| - | width: 910px;
| + | |
| - | /*width: 100%*/
| + | |
| - | margin: 0;
| + | |
| - | border-top: 0;
| + | |
| - | border-right: 0;
| + | |
| - | border-left: 0;
| + | |
| - | padding: 0;
| + | |
| - | background: blue;
| + | |
| - |
| + | |
| - | }
| + | |
| - | div.MenuBar ul {
| + | |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | text-align: center;
| + | |
| - | list-style-type: none;
| + | |
| - | margin: 0.5em auto;
| + | |
| - | border: 0;
| + | |
| - | padding: 0;
| + | |
| - | background: blue;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li {
| + | |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | display: block;
| + | |
| - | padding: 0;
| + | |
| - | margin: 0;
| + | |
| - | font-size: 1.3em;
| + | |
| - | float: left;
| + | |
| - | background: blue;
| + | |
| - | text-align: center;
| + | |
| - | width: 107px;
| + | |
| - | position: relative; /* Sets the positioning context for each drop-down menu. */
| + | |
| - | }
| + | |
| - | | + | |
| - | div.MenuBar ul li a {
| + | |
| - | font: arial, helvetica, sans-serif;
| + | |
| - | display: block;
| + | |
| - | background: blue;
| + | |
| - | height: 22px; /* Keep height + padding-top + padding-bottom sync with the menu bar height. */
| + | |
| - | color: #ffffff;
| + | |
| - | padding-top: 4px;
| + | |
| - | padding-bottom: 4px;
| + | |
| - | padding-left: 1em; /* Sets the left space between top-level items. */
| + | |
| - | padding-right: 1em; /* Sets the right space between top-level items. */
| + | |
| - | text-decoration: none;
| + | |
| - | }
| + | |
| - | | + | |
| - | /*------------------------------------------------------------------------------ Drop-Down Menus */
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
| - | display: block;
| + | |
| - | width: 10em; /* Drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | height: 1em;
| + | |
| - | padding: 1px; /* Sets the drop-down menu "effective border" width. */
| + | |
| - | position: absolute;
| + | |
| - | top: 23px; /* Places the drop-down menu under the menu bar.
| + | |
| - | Keep it sync with the menu bar height. */
| + | |
| - | left: 0; /* Aligns the drop-down menu to its top-level item. */
| + | |
| - | background-color: blue; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | | + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
| - | width: 10em; /* Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | height: 1em;
| + | |
| - | padding-left: 0;
| + | |
| - | padding-right: 0;
| + | |
| - | background-color: blue; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | ul.DropDownMenu li a span {
| + | |
| - | display: block;
| + | |
| - | padding-left: 0.75em; /* Sets the left space of each drop-down menu item. */
| + | |
| - | padding-right: 0.25em; /* Sets the right space of each drop-down menu item. */
| + | |
| - | text-align: right; /* Aligns the >> symbol to the right. */
| + | |
| - | }
| + | |
| - | ul.DropDownMenu li a span span {
| + | |
| - | float: left; /* Aligns the text (back) to the left. */
| + | |
| - | font: 12px arial, helvetica, sans-serif; /* Requiblue for IE55. */
| + | |
| - | height: 20px;
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | /*----------------------------------------------------------------------------------- Side Menus */
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
| - | display: block;
| + | |
| - | width: 11em; /* Side menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | padding: 1px; /* Sets the side menu "effective border" width. */
| + | |
| - | position: absolute;
| + | |
| - | top: -1px; /* Aligns the side menu to its drop-down menu item.
| + | |
| - | Keep it sync with the side menu "effective border" width. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | width: 11em; /* Keep it sync with the side menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | font: 12px arial, helvetica, sans-serif; /* Requiblue for IE55. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li ul.DropDownMenu li ul.SideMenu li a span {
| + | |
| - | padding-left: 1.5em; /* Sets the left space of each side menu item. */
| + | |
| - | padding-right: 0.5em; /* Sets the right space of each side menu item. */
| + | |
| - | text-align: left;
| + | |
| - | font: 12px arial, helvetica, sans-serif; /* Requiblue for IE55. */
| + | |
| - | left: 13em; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width.
| + | |
| - | Use MenuTailor.css to customize. */
| + | |
| - | }
| + | |
| - | /*----------------------------------------------------------------------------- Browser Specific */
| + | |
| - | * html div.MenuBar ul li a {
| + | |
| - | float: left; /* Requiblue for IE55 and IE6.
| + | |
| - | Breaks O9.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | * html ul.DropDownMenu li a:hover {
| + | |
| - | cursor: hand; /* Requiblue for IE55.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | ul.DropDownMenu li a:hover {
| + | |
| - | cursor: pointer; /* Requiblue for IE6 and IE7.
| + | |
| - | Hidding it (* html) from non-IE browsers breaks IE7!
| + | |
| - | }
| + | |
| - | * html div.MenuBar a:hover {
| + | |
| - | text-decoration: none; /* Requiblue for IE55 and IE6.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | * html div.MenuBar ul li table,
| + | |
| - | * html div.MenuBar ul li table td {
| + | |
| - | border: 0; /* Requiblue for IE55 and IE6.
| + | |
| - | Hidden (* html) from non-IE browsers. */
| + | |
| - | }
| + | |
| - | /*------------------------------------------------------------------------------- Default Colors */
| + | |
| - | div.MenuBar {
| + | |
| - | background-color: Menu;
| + | |
| - | border-bottom: 1px solid ButtonShadow;
| + | |
| - | }
| + | |
| - | div.MenuBar a {
| + | |
| - | background-color: Menu; /* Top-level unselected items. */
| + | |
| - | color: MenuText;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover a,
| + | |
| - | div.MenuBar ul li a:hover {
| + | |
| - | color: #ea7f16;
| + | |
| - | background-color: Highlight; /* Top-level selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the drop-down menu "effective border" color. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a {
| + | |
| - | background-color: Menu; Drop-down menu unselected items.
| + | |
| - | Sets the drop-down menu "effective background" color. */
| + | |
| - | color: MenuText;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover {
| + | |
| - | background-color: Highlight; /* Drop-down menu selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the side menu "effective border" color. */
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | background-color: Menu; /* Side menu unselected items.
| + | |
| - | Sets the side menu "effective background" color. */
| + | |
| - | color: MenuText;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
| - | background-color: Highlight; /* Side menu selected item. */
| + | |
| - | color: HighlightText;
| + | |
| - | }
| + | |
| - | /*-----------------------------------------------------------------------------------------------*/
| + | |
| - | /*Script-Free 3-Level Menu 1.2 Tailor
| + | |
| - | www.CesarDaniel.info
| + | |
| - | /*-------------------------------------------------------------------------------------- General */
| + | |
| - | body {
| + | |
| - | background:url(https://static.igem.org/mediawiki/2010/2/20/Clock.jpg) repeat top left;
| + | |
| - | color: blue;
| + | |
| - | margin: 0;
| + | |
| - | border: 0;
| + | |
| - | padding: 0;
| + | |
| - | }
| + | |
| - | | + | |
| - | | + | |
| - | div.MenuBar {
| + | |
| - | font: 13px arial, helvetica, sans-serif;
| + | |
| - | }
| + | |
| - | div.MenuBar ul {
| + | |
| - | font: 13px arial, helvetica, sans-serif; /* Requiblue for IE55. */
| + | |
| - | }
| + | |
| - | /*--------------------------------------------------------------------------------------- Colors */
| + | |
| - | div.MenuBar {
| + | |
| - | background-color: blue; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | border-bottom: 1px solid ButtonShadow;
| + | |
| - | }
| + | |
| - | div.MenuBar a,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a {
| + | |
| - | background-color: blue; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover a,
| + | |
| - | div.MenuBar ul li a:hover,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover a,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu li a:hover,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu li a:hover {
| + | |
| - | background-color: #00b0e6; /* Selected item. */
| + | |
| - | color: #FFFFFF;
| + | |
| - | }
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu,
| + | |
| - | div.MenuBar ul li:hover ul.DropDownMenu li:hover ul.SideMenu,
| + | |
| - | div.MenuBar ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu {
| + | |
| - | background-color: ButtonShadow; /* Sets the drop-down and side menus "effective border" color. */
| + | |
| - | }
| + | |
| - | /*--------------------------------------------------------------------------------------- Widths */
| + | |
| - | /*
| + | |
| - | | + | |
| - | /*
| + | |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
| + | |
| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM4 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM4 li a {
| + | |
| - | width: 11em; /* Drop-down menu width. */
| + | |
| - | }
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu#MB1-DDM5 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu#MB1-DDM5 li a {
| + | |
| - | width: 12em; /* Drop-down menu width. */
| + | |
| - | }
| + | |
| - | | + | |
| - | /*...............................................................................................*/
| + | |
| - | /*
| + | |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
| + | |
| - | Side Menu #1
| + | |
| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 {
| + | |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width. */
| + | |
| - | }
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM1 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM1 li a {
| + | |
| - | width: 10em; /* Side menu width. */
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | /*
| + | |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
| + | |
| - | Side Menu #2
| + | |
| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 {
| + | |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width. */
| + | |
| - | }
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM2 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM2 li a {
| + | |
| - | width: 10em; /* Side menu width. */
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | /*
| + | |
| - | Menu Bar 1
| + | |
| - | Drop-Down Menu #2
| + | |
| - | Side Menu #3
| + | |
| - | */
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 {
| + | |
| - | left: 15.5em !important; /* Places the side menu to the right of the drop-down menu.
| + | |
| - | Keep it sync with the drop-down menu width. */
| + | |
| - | }
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3,
| + | |
| - | div.MenuBar#navi ul li:hover ul.DropDownMenu li:hover ul.SideMenu#MB1-DDM2-SM3 li a,
| + | |
| - | div.MenuBar#navi ul li a:hover ul.DropDownMenu li a:hover ul.SideMenu#MB1-DDM2-SM3 li a {
| + | |
| - | width: 10em; /* Side menu width. */
| + | |
| - | }
| + | |
| - | /*...............................................................................................*/
| + | |
| - | | + | |
| - | </style>
| + | |
| - | | + | |
| - | <body>
| + | |
| - | <div id="header"><img src="https://static.igem.org/mediawiki/2010/e/e3/Uppsala_universitet_medium.jpg" alt="Team Uppsala" height="150" width="150" /><img src="https://static.igem.org/mediawiki/igem.org/5/54/Uppsala_480257b.jpg" alt="Team Uppsala" height="150" width="756" /></div>
| + | |
| - | <div class='MenuBar' id="navi">
| + | |
| - | <ul>
| + | |
| - | <li>
| + | |
| - | <a href="https://2010.igem.org/Team:Uppsala-Sweden" style="color: white">Home
| + | |
| - | <!--[if gt IE 6]><!--></a><!--<![endif]-->
| + | |
| - | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
| + | |
| - | <!--[if lte IE 6]></td></tr></table></a><![endif]-->
| + | |
| - | </li>
| + | |
| - | <li>
| + | |
| - | <a href="https://2010.igem.org/Team:Uppsala-SwedenTeam" style="color: white">Team<!--[if gt IE 6]><!--></a><!--<![endif]-->
| + | |
| - | <!--[if lt IE 7]><table border="0" cellpadding="0" cellspacing="0"><tr><td><![endif]-->
| + | |
| - | <ul class="DropDownMenu" id="MB1-DDM6">
| + | |
| - | <li><a href="https://2010.igem.org/Team:Uppsala-SwedenTeam"><span><span>Overview</span></span></a></li>
| + | |
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| | == Week-1 (Brainstorming) == | | == Week-1 (Brainstorming) == |
| | | | |
| - | == Ideas discussed Before choosing topic: ==
| + | This was a fresh first week. It’s so cool to have an international team like us. We are so sure to generate many novel and interest ideas. After several brainstorm meeting, 12 innovative project proposals was freshly born. All idea are compared and discussed by their advantages and disadvantages but finally only 2 were selected to be further studied. |
| | | | |
| - | (1) Thermometer, change color with temp (10-45 degree), E.coli express 2 color proteins in different ratio under various temp
| + | '''1.Thermometer''' |
| | | | |
| - | (2) singing e.coli E.coli produce magnetosome--so create magnetic field ---vibration-- amplify-- sound!!
| + | This would be a practical project as it develops a common device in our daily life and the idea is quite simple. We plan to insert 2 colour protein genes in to E.coli and those genes are controlled by temperature sensitive sensors. The designed cells can display different colours under various temperatures within 10 to 45. Our idea is based on a related project by UCSF team in 2005 [https://2006.igem.org/UCSF_2005 i]. With the help of several cold and heat shock sensors that UCSF team has synthesized, we could improve the sensitivity of the sensor and make a more instant thermometer with colour indication. |
| | | | |
| - | (3) Compass, also e.coli produce magnetosome-- line up to
| + | '''2.Singing E.coli''' |
| | | | |
| - | (4) Post-modern arts E.coli produce different color(fluorescent) protein under different conditions(temp, pH, growth
| + | It would be super cool if we could hear the sound of E.coli, right? What a crazy idea! But we are going to make impossible happen. The project is based on researches on a kind of special bacteria called magnetotactic bacteria. Those microorganisms are widely existed in condensation of fresh and salty water and play important role in circulation of iron. This specie can move to the North point of magnet since containing a magnetic protein complex called magnetosome [https://www.annualreviews.org/doi/pdf/10.1146/annurev.mi.36.100182.001245 ii]. This magnetic property is a hot topic in recently years and could be used in many area of industry. However, those magetotactic bacteria are mostly anaerobic and hard to cultivate. We could introduce magnetosome into E.coli and grow those special cells in a tank. With the help of rapid changing magnetic field outside of tank, the magnetosome will bring the E.coli cells to vibrate and the motion could be amplified by electronic speaker. Well, it won’t be a beautiful song but it would be so cool to hear E.coli. |
| - | level,oxygen conc...) the colors will change randomly and show our *post modern* picture
| + | |
| | | | |
| - | (5) Electricity generationg E.coli, produce 2 kind of charged e.coli culture, electron in the buffer will moving and
| + | '''3.Heavy metal detector''' |
| - | electrical potiential will be created.
| + | |
| | | | |
| - | (6) e.coli plasma* produce different color, but in the controlled way to show some pic.
| + | Another practical project! This project is also going to use E.coli transformed with magnetosome but for different purpose. Based on the result from a research group in University of Southampton [http://www.informaworld.com/smpp/content~db=all?content=10.1081/SS-120014825 iii], with the magnetic property, the bacteria could attract and remove metal ions like Fe, Mg, Mn, Al and Gd in the environment. It would be an economical, clean and energy-saving method to protect the nature from pollution. Our project will be creating a heavy metal detector by introducing the magnetosome and coloured proteins genes in E.coli. With contact to heavy metal, the bacteria will show various colour indicating the concentration in the waste water. |
| | | | |
| - | (7) Snow melting e.coli product(especially useful in sweden): e.coli will grow in low temperature and the protein will melt
| + | '''4.Post-modern painting''' |
| - | the snow, Great idea for Sweden. coz swedish government use a lot of money to gather the snow in one particular field and wait
| + | |
| - | the snow to melt. this will save a lot of time to melting the snow. and it could be possible the protein is useful in other
| + | |
| - | way.
| + | |
| | | | |
| - | (8) Cleaning agent - Express proteases, lipases etc.. in same microbe. Allows for cleaning of organic matter from objects like clothes and utensils.
| + | Do you want a post-modern painting which changing the pattern all the time on your wall? This project aims to generate a kind of E.coli cells which could display different colour according to different conditions such as temp, pH, oxygen concentration, and electric potentials etc. We will randomly alter the conditions on each small area of the picture time by time. The colour protein genes will be borrowed from last year’s iGEM team from Cambridge iv. If the works goes quite smooth, we could try to control the colours on each spot and create a bacteria plasma screen which could show words or simple pictures. |
| | | | |
| - | (9) BioAmplifier - Detects low amounts of input signal and amplifies it while maintaining intensity. Can be very useful in Amplifying Intenstiy specific values.
| + | '''5.Snow and ice melter''' |
| | | | |
| - | (10) Variable Regulator - Allows for changing a system for different values. Ex: Volume control knob on a sterio.
| + | In the country such as Sweden, the first impression of winter is everything covering by snow and every year the government put great efforts to remove snow on the streets and roofs. Moreover, icy ground is also a big trouble for walking and riding in cold winter. It would save a lot of money if the snow and ice could melt quickly and won’t refreeze even under low temperature. The existing melter products are mainly consists of chloride salts which are a pollutant if widely used. So our team want to invent an organic snow and ice melter by synthetic biology. A special kind of bacteria is the main component in the melter which could gain energy from the sunlight efficiently and melt the ice in a short time. After finish its work in one spot, the bacteria will move to on with the melted water. |
| | | | |
| - | (11) Display device - Selective expression of colored proteins to display characters(alphabets or numbers).The character to be
| + | '''6.Bioamplifier''' |
| - | displayed is given as input by a protein. It will be possible to write word by just adding a drop of the protein for each
| + | |
| - | letter.
| + | |
| | | | |
| - | (12) Bio Timer - a timing device which can produce a time delayed signal. The amount of delay is regulated by the input signal. Possible to build a clock based on this? Compare with mechanical clock?
| + | A bioamplifier could help us to amplify the intensity of some signals that detected. According to the 2007 iGEM team from Cambridge [https://2007.igem.org/Cambridge v], a, so called, PoPS amplifier was built to increase the strength of the signalling system. However, we think we could improve the amplifier to be more sensitive and more specific and introduce the various colours indicating gradients of signal intensity. The project could be quite practical and work as a tool for future iGEM teams. A further improvement could be adding a control of the amount of colour intensity increased corresponding to the signal it received. A simple example could be the volume control knob on the stereo. |
| | | | |
| | + | '''7.Display device''' |
| | | | |
| | + | This project also involves quorum sensing system and the colour proteins mentioned by last year’s iGEM team from Cambridge [https://2009.igem.org/Team:Cambridge iv]. We will generate several different kinds of E.coli type and cultivate them in array in certain order. So when a specific chemical diffused into each culture, some culture might have no response at all but others might express a coloured protein accordingly. The coloured colonies as a whole will display a given character, such as alphabets or numbers. But if another chemical is involved, it might cause a different expression of proteins in some other colonies, and then a different character will be shown. |
| | | | |
| - | We brainstormed about the advantages and disadvantages of going with each of the different project ideas and finalised few of the ideas for further work.Many of the ideas had already been implemented by previous iGEM teams. After researching the ideas we had further discussions about choosing a single topic to work on.
| + | '''8.Bio Timer''' |
| | | | |
| - | Outcome:We decided to go ahead with building a Bio-Clock which displays time either by colour or numerically.
| + | Could your timer in the kitchen be a biological based device? This project aims to design a Bio timer using quorum sensing and band detection based on a research group in Princeton University [http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html vi]. A general idea is to cultivate 12 colonies on a plate, and the timer will be initiated by adding some AHL. After one hour, the first colony will show colour and produce more AHL sending to the next colony. The band detection technique prevents the backward of signal detection. So, the bio timer could show hour by hour difference for half day. It could be improved if time measurement is based on oscillation instead of diffusion of signalling substances [http://www.ncbi.nlm.nih.gov/pubmed/10659857 vii]. |
| - | |}
| + | |
This was a fresh first week. It’s so cool to have an international team like us. We are so sure to generate many novel and interest ideas. After several brainstorm meeting, 12 innovative project proposals was freshly born. All idea are compared and discussed by their advantages and disadvantages but finally only 2 were selected to be further studied.
This would be a practical project as it develops a common device in our daily life and the idea is quite simple. We plan to insert 2 colour protein genes in to E.coli and those genes are controlled by temperature sensitive sensors. The designed cells can display different colours under various temperatures within 10 to 45. Our idea is based on a related project by UCSF team in 2005 i. With the help of several cold and heat shock sensors that UCSF team has synthesized, we could improve the sensitivity of the sensor and make a more instant thermometer with colour indication.
It would be super cool if we could hear the sound of E.coli, right? What a crazy idea! But we are going to make impossible happen. The project is based on researches on a kind of special bacteria called magnetotactic bacteria. Those microorganisms are widely existed in condensation of fresh and salty water and play important role in circulation of iron. This specie can move to the North point of magnet since containing a magnetic protein complex called magnetosome ii. This magnetic property is a hot topic in recently years and could be used in many area of industry. However, those magetotactic bacteria are mostly anaerobic and hard to cultivate. We could introduce magnetosome into E.coli and grow those special cells in a tank. With the help of rapid changing magnetic field outside of tank, the magnetosome will bring the E.coli cells to vibrate and the motion could be amplified by electronic speaker. Well, it won’t be a beautiful song but it would be so cool to hear E.coli.
Another practical project! This project is also going to use E.coli transformed with magnetosome but for different purpose. Based on the result from a research group in University of Southampton [http://www.informaworld.com/smpp/content~db=all?content=10.1081/SS-120014825 iii], with the magnetic property, the bacteria could attract and remove metal ions like Fe, Mg, Mn, Al and Gd in the environment. It would be an economical, clean and energy-saving method to protect the nature from pollution. Our project will be creating a heavy metal detector by introducing the magnetosome and coloured proteins genes in E.coli. With contact to heavy metal, the bacteria will show various colour indicating the concentration in the waste water.
Do you want a post-modern painting which changing the pattern all the time on your wall? This project aims to generate a kind of E.coli cells which could display different colour according to different conditions such as temp, pH, oxygen concentration, and electric potentials etc. We will randomly alter the conditions on each small area of the picture time by time. The colour protein genes will be borrowed from last year’s iGEM team from Cambridge iv. If the works goes quite smooth, we could try to control the colours on each spot and create a bacteria plasma screen which could show words or simple pictures.
In the country such as Sweden, the first impression of winter is everything covering by snow and every year the government put great efforts to remove snow on the streets and roofs. Moreover, icy ground is also a big trouble for walking and riding in cold winter. It would save a lot of money if the snow and ice could melt quickly and won’t refreeze even under low temperature. The existing melter products are mainly consists of chloride salts which are a pollutant if widely used. So our team want to invent an organic snow and ice melter by synthetic biology. A special kind of bacteria is the main component in the melter which could gain energy from the sunlight efficiently and melt the ice in a short time. After finish its work in one spot, the bacteria will move to on with the melted water.
A bioamplifier could help us to amplify the intensity of some signals that detected. According to the 2007 iGEM team from Cambridge v, a, so called, PoPS amplifier was built to increase the strength of the signalling system. However, we think we could improve the amplifier to be more sensitive and more specific and introduce the various colours indicating gradients of signal intensity. The project could be quite practical and work as a tool for future iGEM teams. A further improvement could be adding a control of the amount of colour intensity increased corresponding to the signal it received. A simple example could be the volume control knob on the stereo.
This project also involves quorum sensing system and the colour proteins mentioned by last year’s iGEM team from Cambridge iv. We will generate several different kinds of E.coli type and cultivate them in array in certain order. So when a specific chemical diffused into each culture, some culture might have no response at all but others might express a coloured protein accordingly. The coloured colonies as a whole will display a given character, such as alphabets or numbers. But if another chemical is involved, it might cause a different expression of proteins in some other colonies, and then a different character will be shown.
Could your timer in the kitchen be a biological based device? This project aims to design a Bio timer using quorum sensing and band detection based on a research group in Princeton University [http://www.nature.com/nature/journal/v434/n7037/full/nature03461.html vi]. A general idea is to cultivate 12 colonies on a plate, and the timer will be initiated by adding some AHL. After one hour, the first colony will show colour and produce more AHL sending to the next colony. The band detection technique prevents the backward of signal detection. So, the bio timer could show hour by hour difference for half day. It could be improved if time measurement is based on oscillation instead of diffusion of signalling substances [http://www.ncbi.nlm.nih.gov/pubmed/10659857 vii].
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