Team:Newcastle/Slide Preparation
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+ | #Grow overnight culture in 5ml of LB broth at 37C | ||
+ | #Dilute the overnight culture to a ratio of 1:100 and incubate at 37C for 1 hour | ||
+ | #Set up the following broths with different concentrations of IPTG in 5ml of LB broth | ||
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+ | * 2mM IPTG | ||
+ | * 0.2mM IPTG | ||
+ | * 0.02mM IPTG | ||
+ | * Broth only | ||
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+ | {{Team:Newcastle/footer}} |
Revision as of 08:24, 23 September 2010
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- Grow overnight culture in 5ml of LB broth at 37C
- Dilute the overnight culture to a ratio of 1:100 and incubate at 37C for 1 hour
- Set up the following broths with different concentrations of IPTG in 5ml of LB broth
- 2mM IPTG
- 0.2mM IPTG
- 0.02mM IPTG
- Broth only
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