Team:RMIT Australia/Notebook/Media
From 2010.igem.org
(Difference between revisions)
(New page: __NOTOC__ <html> <body style="background-color:#CCCCCC"> <style> h1.firstHeading { display: none; } p {text-align: justify;} a:link { color: #FF0000; text-decoration: none} a:visited {...) |
(→Media Recipes) |
||
(4 intermediate revisions not shown) | |||
Line 431: | Line 431: | ||
=='''Media Recipes '''== | =='''Media Recipes '''== | ||
- | '''SOC Medium''' | + | '''SOC Medium'''<br> |
- | 4g Tryptone | + | 4g Tryptone<br> |
- | 1g Yeast extract | + | 1g Yeast extract<br> |
- | 500mg NaCl | + | 500mg NaCl<br> |
- | 2ml 250mM KCl | + | 2ml 250mM KCl<br> |
- | Deionised water up to 190ml | + | Deionised water up to 190ml<br> |
Then autoclave | Then autoclave | ||
- | '''LB Media''' | + | |
- | 5g Tryptone | + | <p> |
- | 2.5 g yeast extract | + | '''LB Media'''<br> |
- | 5g | + | 5g Tryptone<br> |
- | 500ml deionised water | + | 2.5 g yeast extract<br> |
+ | 5g NaCl<br> | ||
+ | 500ml deionised water <br> | ||
Then autoclave | Then autoclave |
Latest revision as of 04:45, 23 September 2010
![](https://static.igem.org/mediawiki/2010/3/31/RMIT_logo.png)
![](https://static.igem.org/mediawiki/2008/e/ef/Navi_bg.gif)
Media Recipes
SOC Medium
4g Tryptone
1g Yeast extract
500mg NaCl
2ml 250mM KCl
Deionised water up to 190ml
Then autoclave
LB Media
5g Tryptone
2.5 g yeast extract
5g NaCl
500ml deionised water
Then autoclave