Team:Tokyo Metropolitan/Notebook/Pattern/2010/09/06

From 2010.igem.org

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<Protocol>
<Protocol>
-
See Protocol10
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See [https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#DNA_Ligation_with_Ligation-convenience_kit_.28NIPPON_GENE.29/ Protocol 3 ]

Revision as of 10:44, 22 September 2010

Contents

2010/09/06

1:PCR

<Member>

Hitomi,nito


<Sample>

  • 1(BBa_K208017)
  • 2(BBa_JM109)
  • 5(BBa_K208017)


<Protocol>

See Protocol2

(https://2010.igem.org/Team:Tokyo_Metroporitan/Project/Pattern/Protocol#PCR_with_Tag_DNA_Polymerase_.28NIPPON_GENE.29/)


Tubes(Temperature in annealing)

  • 1(BBa_K208017) 71.5,72.0,72.5℃
  • 2(BBa_JM109) 71.0,71.5,72.0℃
  • 5(BBa_K208017) 70.0,70.5,71.0℃

2:Purification of PCR

<Member>

Hitomi


<Protocol>

See Protocol

3:Electrophoresis

<Samlpe>

PCR products.

  • 1
  • 2
  • 5
  • 7
  • 8


<Protocol>

See Protocol8

(https://2010.igem.org/Team:Tokyo_Metropolitan/Project/Pattern/Protocol#Electrophoresis/)


<Result>

File:Matsuura 2010-09-06 16hr 49min.tif

4:Ligation

<Membber>

nito


<Sample>

  • 7
  • 8


<Protocol>

See Protocol 3

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