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Revision as of 05:05, 17 September 2010 (view source) Jocelynatustc (Talk | contribs) (New page: Jocelyn: colony PCR to test pduN. colony PCR before miniprep ×40 double digest of ABJKTUV in promoter and pduN. storage of bacterial ×36) ← Older edit		Latest revision as of 09:49, 22 September 2010 (view source) Hildegard (Talk | contribs)
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	storage of bacterial ×36		storage of bacterial ×36
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		+	----
		+	Hilde:
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		+	PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL
		+
		+	PCR test of pduN (N-r, N-f) Model: NTUV plm.-2/-3; NTUV plm.2 No.4-1-2-3; ABJKNTUV#2-#9-#5;TUV5(AD);N(+);NULL
		+
		+	Fastdigest cutting: pduN (Insert,X,P) & Promotor (Vector,S,P)
		+
		+	miniprep: NU1-8;JK1-1-8;RBS1-2
		+
		+	Sending to sequence test: JK1-4-7;NU1-4-7;RBS1-2

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Latest revision as of 09:49, 22 September 2010

Jocelyn:

colony PCR to test pduN.

colony PCR before miniprep ×40

double digest of ABJKTUV in promoter and pduN.

storage of bacterial ×36

---

Hilde:

PCR test of pduN (N-r, N-f) Model: NTUV plm.-1/-2/-3; NTUV plm.2 No.1-2-3-4; ABJKNTUV#2-#9-#5;TUV5(AD);NULL

PCR test of pduN (N-r, N-f) Model: NTUV plm.-2/-3; NTUV plm.2 No.4-1-2-3; ABJKNTUV#2-#9-#5;TUV5(AD);N(+);NULL

Fastdigest cutting: pduN (Insert,X,P) & Promotor (Vector,S,P)

miniprep: NU1-8;JK1-1-8;RBS1-2

Sending to sequence test: JK1-4-7;NU1-4-7;RBS1-2