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Team:USTC/Notebook/Meetings/12 September 2010
From 2010.igem.org
(Difference between revisions)
(New page: Jocelyn: Double Digest pduA in RFC 53 standard.=> ligate pduA into pduB. Transformation of biobrick promoter-pdu full length into trans 10 => amplify the signal colony from the LB plate.) |
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Jocelyn: | Jocelyn: | ||
| - | Double Digest pduA in RFC | + | Double Digest pduA in new BBF RFC standard.=> ligate pduA into pduB. |
Transformation of biobrick promoter-pdu full length into trans 10 => amplify the signal colony from the LB plate. | Transformation of biobrick promoter-pdu full length into trans 10 => amplify the signal colony from the LB plate. | ||
| + | |||
| + | ---- | ||
| + | Hilde: | ||
| + | |||
| + | Transformation of GFP & RFP | ||
| + | |||
| + | Coating plm. of GFP onto A+ plate | ||
| + | |||
| + | Coating plm. of RFP onto Cl+ plate | ||
| + | |||
| + | Fastdigest cutting of pduABJKNTUV(X,P) & Promotor (S,P) for 3 hours | ||
| + | |||
| + | DNA ligation Kit LONG: Promotor-pduABJKNT (overnight) | ||
Latest revision as of 09:21, 22 September 2010
Jocelyn:
Double Digest pduA in new BBF RFC standard.=> ligate pduA into pduB.
Transformation of biobrick promoter-pdu full length into trans 10 => amplify the signal colony from the LB plate.
Hilde:
Transformation of GFP & RFP
Coating plm. of GFP onto A+ plate
Coating plm. of RFP onto Cl+ plate
Fastdigest cutting of pduABJKNTUV(X,P) & Promotor (S,P) for 3 hours
DNA ligation Kit LONG: Promotor-pduABJKNT (overnight)
