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Team:USTC/Notebook/Meetings/5 September 2010
From 2010.igem.org
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Plasmid extration of pdu NT-UV and pdu JK-NU in pSB1C7 | Plasmid extration of pdu NT-UV and pdu JK-NU in pSB1C7 | ||
| + | |||
| + | |||
| + | ------------ | ||
| + | Knightmare: | ||
| + | |||
| + | Liuchang's colony PCR^^ | ||
| + | |||
| + | ---- | ||
| + | Hilde: | ||
| + | |||
| + | Transformation of Ligation product of Promotor+pduABJKNTUV | ||
| + | |||
| + | Coating the transformation product of Promotor+pduABJKNTUV to Cl+ plate | ||
| + | |||
| + | Selection of single colony of ProABJKNTUV | ||
| + | |||
| + | Colony PCR test(VR2->ABr3) | ||
Latest revision as of 07:37, 22 September 2010
Evelyn:
Transformation of the ligation product: pduAB-JK.
LiuChang:
Plasmid extration of pdu NT-UV and pdu JK-NU in pSB1C7
Knightmare:
Liuchang's colony PCR^^
Hilde:
Transformation of Ligation product of Promotor+pduABJKNTUV
Coating the transformation product of Promotor+pduABJKNTUV to Cl+ plate
Selection of single colony of ProABJKNTUV
Colony PCR test(VR2->ABr3)
