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From 2010.igem.org

Evelyn:

Transformation of the ligation product: pduAB-JK.

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LiuChang:

Plasmid extration of pdu NT-UV and pdu JK-NU in pSB1C7

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Knightmare:

Liuchang's colony PCR^^

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Hilde:

Transformation of Ligation product of Promotor+pduABJKNTUV

Coating the transformation product of Promotor+pduABJKNTUV to Cl+ plate

Selection of single colony of ProABJKNTUV

Colony PCR test(VR2->ABr3)