Team:Tokyo Metropolitan/Notebook/Fiber/2010/08/16

From 2010.igem.org

(Difference between revisions)
(Experiment:Agarose Gel Electro-phoresis)
(Experiment:Agarose Gel Electro-phoresis)
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'''Procedure'''
'''Procedure'''
-
#Pour the Agarose gel in a box and make dents on it
+
#Pour the Agarose gel in a box and make wells on it
-
#Pour the 10×Loading buffer in the one of dents
+
#Pour the 10×Loading buffer in the one of wells
-
#Pour the Insert DNA in another dent.
+
#Pour the Insert DNA in another well.
'''Consequence'''<br />
'''Consequence'''<br />

Revision as of 06:19, 21 September 2010

2010/08/16 Monday(NEX)

Experiment:Make Agarose Gels Electro-phoresis

Member
NEX and watachin

Materials

  • 1×TaE 200ml
  • Agarose 2g
  • Ethidium bromide 10µl

Procedure

  1. Add and mixture above materials in an Erlenmeyer flask
  2. Warm up them in a Microwave oven

Experiment:Agarose Gel Electro-phoresis

Member
Same avobe

Materials

  • Insert DNA
  • Agarose Gel
  • 10×Loading buffer 5μl

Procedure

  1. Pour the Agarose gel in a box and make wells on it
  2. Pour the 10×Loading buffer in the one of wells
  3. Pour the Insert DNA in another well.

Consequence
We could see the bcsB.

20100816.jpg