Team:Lethbridge/Notebook/Working Plasmids

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Latest revision as of 22:03, 20 September 2010

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Updated June 1/2010

Row A

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

A1 sRBS-Lum-dT(2) May 13/2010 (JV) 965

A2 sRBS-Lum-dT(1) May 13/2010 (JV) 1145

A3 Lumazine May 13/2010 (AV) 750

A4 pBad(1) May 13/2010 (AV) 1320

A5 pBad(2) May 13/2010 (JV) 2050

A6 sRBS May 15/2010 6970

A7 sRBS May 15/2010 17000

A8 CFP Complete Feb 20/2010 1395

A9 pLacI Feb 20/2010 990

A10 sRBS May 18/2010 1765

Row B

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

B1 EYFP May 13/2010 1370

B2 N-term tag May 13/2010 415

B3

B4 pSB-NEYFP May 13/2010 1185

B5 pSB-CEYFP May 13/2010 1055

B6 CFP Complete May 13/2010 1285

B7 sRBS-Lum-dT May 13/2010 4375

B8 sRBS-Lum-dT Feb 20/2010 4780

B9 mr. Gene mms6 Feb 20/2010 2455

B10 pBad-TetR May 18/2010 2915

Row C

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

C1 dT 2915

C2

C3

C4

C5

C6

C7

C8

C9

C10

Row D

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

D1 pLacI 70

D2 sRBS-Lum-dT 95

D3 C-term (H4) 860

D4 C-term (I4) 1145

D5 C-term (J4) 1805

D6 pLacI (B1) 440

D7 xylE (B4) 1820

D8 xylE (C4) 420

D9 mms6 (A6) 1530

D10 mms6 (B6) 1440

Row E

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

E1 NEYFP 235

E2 NEYFP 2980

E3 Fusion CEYFP 255

E4 Fusion CEYFP 490

E5 Fusion CEYFP 335

E6 CEYFP 1605

E7 CEYFP 1930

E8 EYFP 640

E9 EYFP 605

E10 EYFP 620

Row F

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

F1 ECFP 470

F2 ECFP 1300

F3 ECFP 2010

F4 pBad-TetR 400

F5 pBad-TetR 575

F6

F7

F8

F9

F10

Row G

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

G1 EYFP 1300

G2 sRBS-Lum-dT(1) 335

G3 sRBS-Lum-dT(2) 540

G4 pSB-CEYFP 340

G5 CFP Complete 355

G6 pBad(1) 175

G7 pBad(2) 315

G8 N-term tag 270

G9 Lumazine 265

G10

Row H

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

H1

H2

H3

H4

H5

H6

H7

H8

H9

H10

Row I

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

I1

I2

I3

I4

I5

I6

I7

I8

I9

I10

Row J

Cell Common Name Standard Registry of Parts ID Date Entered Concentration via
Spectroscopy(µg/mL) Concentration via
gel quantification (µg/mL)

J1

J2

J3

J4

J5

J6

J7

J8

J9

J10

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+</th>
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+</body>
+</html>
+<hr>
+<BLOCKQUOTE>
 Back to [[Team:Lethbridge/Notebook|Notebook]]<br><br>
 <b>Updated June 1/2010</b><br><br>
@@ Line 4: / Line 102: @@
 <table><table border="3">
 <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(&micro;g/mL)</td><td>Concentration via <br>gel quantification (&micro;g/mL)</td></tr>
-<tr><td>A1</td><td>sRBS-Lum-dT(2)</td><td></td><td></td><td>965</td><td></td></tr>
+<tr><td>A1</td><td>sRBS-Lum-dT(2)</td><td></td><td>May 13/2010 (JV)</td><td>965</td><td></td></tr>
-<tr><td>A2</td><td>sRBS-Lum-dT(1)</td><td></td><td></td><td>1145</td><td></td></tr>
+<tr><td>A2</td><td>sRBS-Lum-dT(1)</td><td></td><td>May 13/2010 (JV)</td><td>1145</td><td></td></tr>
-<tr><td>A3</td><td>Lumazine</td><td></td><td></td><td>750</td><td></td></tr>
+<tr><td>A3</td><td>Lumazine</td><td></td><td>May 13/2010 (AV)</td><td>750</td><td></td></tr>
-<tr><td>A4</td><td>pBad(1)</td><td></td><td></td><td>1320</td><td></td></tr>
+<tr><td>A4</td><td>pBad(1)</td><td></td><td>May 13/2010 (AV)</td><td>1320</td><td></td></tr>
-<tr><td>A5</td><td>pBad(2)</td><td></td><td></td><td>2050</td><td></td></tr>
+<tr><td>A5</td><td>pBad(2)</td><td></td><td>May 13/2010 (JV)</td><td>2050</td><td></td></tr>
-<tr><td>A6</td><td>sRBS</td><td></td><td></td><td>6970</td><td></td></tr>
+<tr><td>A6</td><td>sRBS</td><td></td><td>May 15/2010</td><td>6970</td><td></td></tr>
-<tr><td>A7</td><td>sRBS</td><td></td><td></td><td>17000</td><td></td></tr>
+<tr><td>A7</td><td>sRBS</td><td></td><td>May 15/2010</td><td>17000</td><td></td></tr>
-<tr><td>A8</td><td>CFP Complete</td><td></td><td></td><td>1395</td><td></td></tr>
+<tr><td>A8</td><td>CFP Complete</td><td></td><td>Feb 20/2010</td><td>1395</td><td></td></tr>
-<tr><td>A9</td><td>pLacI</td><td></td><td></td><td>990</td><td></td></tr>
+<tr><td>A9</td><td>pLacI</td><td></td><td>Feb 20/2010</td><td>990</td><td></td></tr>
-<tr><td>A10</td><td>sRBS</td><td></td><td></td><td>1765</td><td></td></tr>
+<tr><td>A10</td><td>sRBS</td><td></td><td>May 18/2010</td><td>1765</td><td></td></tr>
 </table><br>
 <b>Row B</b>
 <table><table border="3">
 <tr><td>Cell</td><td>Common Name</td><td>Standard Registry of Parts ID</td><td>Date Entered</td><td>Concentration via<br> Spectroscopy(&micro;g/mL)</td><td>Concentration via <br>gel quantification (&micro;g/mL)</td></tr>
-<tr><td>B1</td><td>EYFP</td><td></td><td></td><td>1370</td><td></td></tr>
+<tr><td>B1</td><td>EYFP</td><td></td><td>May 13/2010</td><td>1370</td><td></td></tr>
-<tr><td>B2</td><td>N-term tag</td><td></td><td></td><td>415</td><td></td></tr>
+<tr><td>B2</td><td>N-term tag</td><td></td><td>May 13/2010</td><td>415</td><td></td></tr>
 <tr><td>B3</td><td></td><td></td><td></td><td></td><td></td></tr>
-<tr><td>B4</td><td>pSB-NEYFP</td><td></td><td></td><td>1185</td><td></td></tr>
+<tr><td>B4</td><td>pSB-NEYFP</td><td></td><td>May 13/2010</td><td>1185</td><td></td></tr>
-<tr><td>B5</td><td>pSB-CEYFP</td><td></td><td></td><td>1055</td><td></td></tr>
+<tr><td>B5</td><td>pSB-CEYFP</td><td></td><td>May 13/2010</td><td>1055</td><td></td></tr>
-<tr><td>B6</td><td>CFP Complete</td><td></td><td></td><td>1285</td><td></td></tr>
+<tr><td>B6</td><td>CFP Complete</td><td></td><td>May 13/2010</td><td>1285</td><td></td></tr>
-<tr><td>B7</td><td>sRBS-Lum-dT</td><td></td><td></td><td>4375</td><td></td></tr>
+<tr><td>B7</td><td>sRBS-Lum-dT</td><td></td><td>May 13/2010</td><td>4375</td><td></td></tr>
-<tr><td>B8</td><td>sRBS-Lum-dT</td><td></td><td></td><td>4780</td><td></td></tr>
+<tr><td>B8</td><td>sRBS-Lum-dT</td><td></td><td>Feb 20/2010</td><td>4780</td><td></td></tr>
-<tr><td>B9</td><td>mr. Gene mms6</td><td></td><td></td><td>2455</td><td></td></tr>
+<tr><td>B9</td><td>mr. Gene mms6</td><td></td><td>Feb 20/2010</td><td>2455</td><td></td></tr>
-<tr><td>B10</td><td>pBad-TetR</td><td></td><td></td><td>2915</td><td></td></tr>
+<tr><td>B10</td><td>pBad-TetR</td><td></td><td>May 18/2010</td><td>2915</td><td></td></tr>
 </table><br>
 <b>Row C</b>

Cell	Common Name	Standard Registry of Parts ID	Date Entered	Concentration via Spectroscopy(µg/mL)	Concentration via gel quantification (µg/mL)
A1	sRBS-Lum-dT(2)		May 13/2010 (JV)	965
A2	sRBS-Lum-dT(1)		May 13/2010 (JV)	1145
A3	Lumazine		May 13/2010 (AV)	750
A4	pBad(1)		May 13/2010 (AV)	1320
A5	pBad(2)		May 13/2010 (JV)	2050
A6	sRBS		May 15/2010	6970
A7	sRBS		May 15/2010	17000
A8	CFP Complete		Feb 20/2010	1395
A9	pLacI		Feb 20/2010	990
A10	sRBS		May 18/2010	1765