Team:Cambridge/Gibson/Protocol

From 2010.igem.org

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{{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Gibson Assembly: Introduction}}
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{{:Team:Cambridge/Templates/headerbar|colour=#96d446|title=Gibson Assembly: Protocols}}
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Gibson Assembly is a technique for assembling DNA with short (c. 40 bp) overlapping sequences together.  Since these overlapping regions can be easily added by PCR with primers which have added "flaps", any DNA sequences can be joined by this mechanism.
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== Advantages ==
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== Master mix ==
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* No scar created - useful '''fusion proteins''' and '''adding an RBS''', where scars can be problematic.
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Ingredients
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* Can religate linear DNA to a circle - useful for '''site-directed mutagenesis'''
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* Faster than Biobrick assembly, and works with less DNA.
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Revision as of 18:44, 19 September 2010

Retrieved from "http://2010.igem.org/Team:Cambridge/Gibson/Protocol"