Team:UC Davis/notebook/initiator.html
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<table class="pikachu" width="690px"> | <table class="pikachu" width="690px"> | ||
<tr><td class="kirby"> | <tr><td class="kirby"> | ||
- | <p class="header"><b>The Initiator</b></a><p> | + | <p class="header"><b>Assembly Workplan: The Initiator</b></a><p> |
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+ | <img src="https://static.igem.org/mediawiki/2010/2/29/Initiatorconstruct.jpg"><p> | ||
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+ | Because the initiator is one of the smaller constructs, we have decided to use this as an example to illustrate the utmost basic workplan in greater detail. <p> | ||
<a name="startup"><h1>Starting Up</h1></a> | <a name="startup"><h1>Starting Up</h1></a> | ||
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<img src="https://static.igem.org/mediawiki/2010/e/eb/RBSc0051stop.jpg"><br /><p> | <img src="https://static.igem.org/mediawiki/2010/e/eb/RBSc0051stop.jpg"><br /><p> | ||
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+ | Assembly notes <br /> | ||
+ | <ul> | ||
+ | <li>It is most practical to cut BBa_B0015 as the vector because it is only 130 bp long. It would be hard to see on a gel. </li> | ||
+ | </ul><br /> | ||
<a name="r0082"><h1>'RBS & BBa_C0051 & stop' to BBa_R0082</h1></a> | <a name="r0082"><h1>'RBS & BBa_C0051 & stop' to BBa_R0082</h1></a> | ||
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<img src="https://static.igem.org/mediawiki/2010/3/34/Initiatorplasmid.jpg"><br /> | <img src="https://static.igem.org/mediawiki/2010/3/34/Initiatorplasmid.jpg"><br /> | ||
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+ | Assembly notes | ||
+ | <ul> | ||
+ | <li>The promoter had to be cut as a vector because it is only 108 bp by itself.</li> | ||
+ | </ul><br /> | ||
</td> | </td> |
Latest revision as of 11:35, 19 September 2010
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