Team:UC Davis/notebook/lightsensor2.html

From 2010.igem.org

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         <table class="pikachu" width="690px">
         <table class="pikachu" width="690px">
             <tr><td class="kirby">
             <tr><td class="kirby">
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<p class="header"><b>Light Sensor: Take Two</b></a><p>
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<a name="lightsensor"></a><p class="header"><b>Light Sensor: Take Two</b></a><p>
<img src="https://static.igem.org/mediawiki/2010/f/f7/Lightsensornames.jpg"><p>
<img src="https://static.igem.org/mediawiki/2010/f/f7/Lightsensornames.jpg"><p>
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<p class="header"><b>Construct:</b></a><p>
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<a name="construct"></a><p class="header"><b>Construct:</b></a><p>
<img src="https://static.igem.org/mediawiki/2010/4/4b/Lightsensorpiecesx.jpg"><p>
<img src="https://static.igem.org/mediawiki/2010/4/4b/Lightsensorpiecesx.jpg"><p>
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<p class="header"><b>Assembling Part A</b></a><p>
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<a name="partA"></a><p class="header"><b>Assembling Part A</b></a><p>
<table border=0 margin="0" width="500px" padding:0 margin:0>
<table border=0 margin="0" width="500px" padding:0 margin:0>
  <tr>
  <tr>
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     <li>'2&3' cut w/ XbaI & PstI</li>
     <li>'2&3' cut w/ XbaI & PstI</li>
     </ul>
     </ul>
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  </td>
 +
</tr>
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</table><p>
 +
 +
<a name="partB"></a><p class="header"><b>Assembling Part B</b></a><p>
 +
<table border=0 margin="0" width="500px" padding:0 margin:0>
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<tr>
 +
  <td><img src="https://static.igem.org/mediawiki/2010/6/61/X24.jpg"></td>
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  <td>
 +
    <ul>
 +
    <li>B0034 cut w/ SpeI & PstI</li>
 +
    <li>I15009 cut w/ Xba & PstI</li>
 +
    </ul>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td><img src="https://static.igem.org/mediawiki/2010/d/d4/X247x.jpg"></td>
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  <td>
 +
    <ul>
 +
    <li>'2&4' cut w/ EcoRI & SpeI</li>
 +
    <li>B0015 cut w/ EcoRI & XbaI</li>
 +
    </ul>
 +
  </td>
 +
</tr>
 +
<tr>
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  <td><img src="https://static.igem.org/mediawiki/2010/6/6b/XpartB.jpg"></td>
 +
  <td>
 +
    <ul>
 +
    <li>'2&4&7' cut w/ EcoRI & SpeI</li>
 +
    <li>J23119 cut w/ EcoRI & XbaI</li>
 +
    </ul>
 +
  </td>
 +
</tr>
 +
</table><p>
 +
 +
<a name="partC"></a><p class="header"><b>Assembling Part C</b></a><p>
 +
<table border=0 margin="0" width="500px" padding:0 margin:0>
 +
<tr>
 +
  <td><img src="https://static.igem.org/mediawiki/2010/e/ee/X67.jpg"></td>
 +
  <td>
 +
  <ul>
 +
  <li>I15010 cut w/ EcoRI & SpeI</li>
 +
  <li>B0015 cut w/ EcoRI & Xba</li>
 +
  </ul>
 +
  </td>
 +
</tr>
 +
<tr>
 +
  <td><img src="https://static.igem.org/mediawiki/2010/6/61/XpartC.jpg"></td>
 +
  <td>
 +
  <ul>
 +
  <li>B0034 cut w/ SpeI & PstI</li>
 +
  <li>'6 & 7' cut w/ XbaI & PstI</li>
 +
  </ul>
   </td>
   </td>
  </tr>
  </tr>
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                 <td class="kirby">
                 <td class="kirby">
<ul>
<ul>
-
<li>Light Sensor</li>
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<li><a href="#lightsensor" class="help">Light Sensor</a></li>
 +
<li><a href="#construct" class="help">Construct</a></li>
 +
<li><a href="#partA" class="help">Assembly of Part A</a></li>
 +
<li><a href="#partB" class="help">Assembly of Part B</a></li>
 +
<li><a href="#partC" class="help">Assembly of Part C</a></li>
</ul> </td>
</ul> </td>
               </tr>
               </tr>

Latest revision as of 09:20, 19 September 2010

Light Sensor: Take Two

Construct:

Assembling Part A

  • B0034 cut w/ SpeI & PstI
  • I15008 cut w/ XbaI & PstI
  • I13453 cut w/ SpeI & PstI
  • '2&3' cut w/ XbaI & PstI

Assembling Part B

  • B0034 cut w/ SpeI & PstI
  • I15009 cut w/ Xba & PstI
  • '2&4' cut w/ EcoRI & SpeI
  • B0015 cut w/ EcoRI & XbaI
  • '2&4&7' cut w/ EcoRI & SpeI
  • J23119 cut w/ EcoRI & XbaI

Assembling Part C

  • I15010 cut w/ EcoRI & SpeI
  • B0015 cut w/ EcoRI & Xba
  • B0034 cut w/ SpeI & PstI
  • '6 & 7' cut w/ XbaI & PstI

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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