Team:UC Davis/notebook/lightsensor2.html

From 2010.igem.org

Light Sensor: Take Two

Construct:

Assembling Part A

  • B0034 cut w/ SpeI & PstI
  • I15008 cut w/ XbaI & PstI
  • I13453 cut w/ SpeI & PstI
  • '2&3' cut w/ XbaI & PstI

Assembling Part B

  • B0034 cut w/ SpeI & PstI
  • I15009 cut w/ Xba & PstI
  • '2&4' cut w/ EcoRI & SpeI
  • B0015 cut w/ EcoRI & XbaI
  • '2&4&7' cut w/ EcoRI & SpeI
  • J23119 cut w/ EcoRI & XbaI

Assembling Part C

  • I15010 cut w/ EcoRI & SpeI
  • B0015 cut w/ EcoRI & Xba
  • B0034 cut w/ SpeI & PstI
  • '6 & 7' cut w/ XbaI & PstI

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)