Team:UT-Tokyo/Sudoku experiment
From 2010.igem.org
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[https://2010.igem.org/Team:UT-Tokyo/Sudoku_abstract Abstract] | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_abstract Abstract] | ||
[https://2010.igem.org/Team:UT-Tokyo/Sudoku_construct Construct] | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_construct Construct] | ||
- | + | Lab note | |
- | + | ||
[https://2010.igem.org/Team:UT-Tokyo/Sudoku_result Result] | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_result Result] | ||
- | == ''' | + | == '''Lab note''' == |
- | |||
- | |||
This is an abstract page. | This is an abstract page. | ||
If you can see detail data, please click each protocols. | If you can see detail data, please click each protocols. | ||
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=== Make construct === | === Make construct === | ||
- | Detail protocols about this is [https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook | + | Detail protocols about this is: |
+ | |||
+ | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook_6 June] | ||
+ | |||
+ | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook July] | ||
+ | |||
+ | [https://2010.igem.org/Team:UT-Tokyo/Sudoku_notebook_2 August] | ||
=== Check test === | === Check test === | ||
Line 75: | Line 78: | ||
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006 BBa_J31006] | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006 BBa_J31006] | ||
|| plate1-1N || pSB1A2 || 1191bp | || plate1-1N || pSB1A2 || 1191bp | ||
+ | |- | ||
+ | | 12 || hixC || | ||
+ | [http://partsregistry.org/wiki/index.php?title=Part:BBa_J44000 BBa_J44000] | ||
+ | || plate1-1B || pSB1A2 || 26bp | ||
+ | |- | ||
+ | | 13 || --- || | ||
+ | --- | ||
+ | || --- || --- || --- | ||
+ | |- | ||
+ | | 14 || lox66 || | ||
+ | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I718016 BBa_I718016] | ||
+ | || plate1-17H || pSB1A2 || 34bp | ||
+ | |- | ||
+ | | 15 || rbs-mRFP1-terminator || | ||
+ | [http://partsregistry.org/wiki/index.php?title=Part:BBa_I13507 BBa_I13507] | ||
+ | || plate1-22O || pSB1A2 || 861bp | ||
+ | |- | ||
+ | | 16 || location sequence(old) || | ||
+ | order | ||
+ | || --- || --- || --- | ||
+ | |- | ||
+ | | 17 || pSP6(rev) || | ||
+ | order | ||
+ | || --- || --- || --- | ||
+ | |- | ||
+ | | 18 || lox2272 || | ||
+ | order | ||
+ | || --- || --- || --- | ||
+ | |- | ||
+ | | 19 || loading sequence(rev) || | ||
+ | order | ||
+ | || --- || --- || --- | ||
+ | |- | ||
+ | | 20 || frt || | ||
+ | [http://partsregistry.org/Part:BBa_J61020 BBa_J61020] | ||
+ | || From HQ || pSB1A2 || 34bp | ||
+ | |- | ||
+ | | 21 || hin(true) || | ||
+ | [http://partsregistry.org/Part:BBa_J31000 BBa_J31000] | ||
+ | || From HQ || ??? || 573bp | ||
+ | |- | ||
+ | | 22 || hin(rev) || | ||
+ | --- | ||
+ | || --- || ??? || 573bp | ||
+ | |- | ||
+ | | 23 || flpe || | ||
+ | --- | ||
+ | || --- || --- || about 1.2kbp | ||
+ | |- | ||
+ | | 24 || gfp unit || | ||
+ | --- | ||
+ | || --- || --- || about 1.2kbp | ||
+ | |- | ||
+ | | 25 || flpe(rev) || | ||
+ | --- | ||
+ | || --- || --- || about 1.2kbp | ||
|} | |} | ||
- | |||
- | |||
== Main construct == | == Main construct == | ||
- | [[Image: | + | [[Image:Sudoku_const_Main_5.png|700px|Sudoku's main construct]] |
=== Research of SP6 RNApol cDNA and F plasmid === | === Research of SP6 RNApol cDNA and F plasmid === |
Latest revision as of 01:56, 19 September 2010
Sudoku
Abstract Construct Lab note Result
Lab note
This is an abstract page. If you can see detail data, please click each protocols.
Pre-experiment
Make construct
Detail protocols about this is:
Check test
Check test
Number | Name | Link to BioBrick | Plate coordinate | Vector | Code length |
1 | T7 promoter |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I712074 BBa_I712074] | plate1-6N | pSB1AK8 | 46bp |
2 | rbs |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0030 BBa_B0030] | plate1-1H | pSB1A2 | 15bp |
3 | cre recombinase |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J61047 BBa_J61047] | plate1-5D | pSB1A2 | 1037bp |
4 | double terminator |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014] | plate2-24C | pSB1AK3 | 95bp |
5 | lox66 recombinase site |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718017 BBa_I718017] | plate1-17J | pSB1A2 | 34bp |
6 | Kan resistance (rev) |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31002 BBa_J31002] | plate1-2K | pSB1A2 | 816bp |
7 | rbs (rev) |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B0014 BBa_B0014] | plate1-1J | pSB1A2 | 15bp |
8 | lox71 recombinase site |
order-made | --- | --- | 34bp |
9 | single terminator |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_B1006 BBa_B1006] | plate1-4H | pSB1AK3 | 39bp |
10 | constant express promoter |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J23119 BBa_J23119] | plate1-18A | pSB1A2 | 35bp |
11 | Tet resistance (rev) |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J31006 BBa_J31006] | plate1-1N | pSB1A2 | 1191bp |
12 | hixC |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_J44000 BBa_J44000] | plate1-1B | pSB1A2 | 26bp |
13 | --- |
--- | --- | --- | --- |
14 | lox66 |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I718016 BBa_I718016] | plate1-17H | pSB1A2 | 34bp |
15 | rbs-mRFP1-terminator |
[http://partsregistry.org/wiki/index.php?title=Part:BBa_I13507 BBa_I13507] | plate1-22O | pSB1A2 | 861bp |
16 | location sequence(old) |
order | --- | --- | --- |
17 | pSP6(rev) |
order | --- | --- | --- |
18 | lox2272 |
order | --- | --- | --- |
19 | loading sequence(rev) |
order | --- | --- | --- |
20 | frt |
[http://partsregistry.org/Part:BBa_J61020 BBa_J61020] | From HQ | pSB1A2 | 34bp |
21 | hin(true) |
[http://partsregistry.org/Part:BBa_J31000 BBa_J31000] | From HQ | ??? | 573bp |
22 | hin(rev) |
--- | --- | ??? | 573bp |
23 | flpe |
--- | --- | --- | about 1.2kbp |
24 | gfp unit |
--- | --- | --- | about 1.2kbp |
25 | flpe(rev) |
--- | --- | --- | about 1.2kbp |
Main construct
Research of SP6 RNApol cDNA and F plasmid
Research of SP6 RNApol cDNA and F plasmid
Convert lox71(rev) to lox66(rev)
Convert lox71(rev) to lox66(rev)
Make Hin(rev)
Make Hin(rev)
Revise MS2 gene
Revise MS2 gene
Make location(rev)-rbs(rev)-location(rev)
Make location(rev)-rbs(rev)-location(rev)