Team:Lethbridge/Notebook/Lab Work/June

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==June 2010==
==June 2010==
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===June 1/2010===<br>
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===June 1/2010===
JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in [[Team:Lethbridge/Notebook/Working_Plasmids|working plasmids box]].<br><br>
JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in [[Team:Lethbridge/Notebook/Working_Plasmids|working plasmids box]].<br><br>
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===June 2/2010===<br>
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===June 2/2010===
<b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5&alpha; cells  
<b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5&alpha; cells  
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===June 3/2010===<br>
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===June 3/2010===
<b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br>
<b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br>

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Contents

June 2010

June 1/2010

JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in working plasmids box.


June 2/2010

Objective: Isolate plasmid DNA (BBa_J33204) from DH5α cells

June 3/2010

Objective: Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.