"
Team:Lethbridge/Notebook/Lab Work/June
From 2010.igem.org
Adam.smith4 (Talk | contribs) |
Adam.smith4 (Talk | contribs) (→June 2010) |
||
| Line 42: | Line 42: | ||
===June 1/2010===<br> | ===June 1/2010===<br> | ||
JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in [[Team:Lethbridge/Notebook/Working_Plasmids|working plasmids box]].<br><br> | JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in [[Team:Lethbridge/Notebook/Working_Plasmids|working plasmids box]].<br><br> | ||
| + | |||
| + | |||
| + | ===June 2/2010===<br> | ||
| + | <b>Objective:</b> Isolate plasmid DNA (BBa_J33204) from DH5α cells | ||
| + | |||
| + | ===June 3/2010===<br> | ||
| + | <b>Objective:</b> Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.<br> | ||
Revision as of 00:54, 5 June 2010
| 
|
| ||||||
|---|---|---|---|---|---|---|---|---|
| ||||||||
]
]
]
]
]
]
June 2010
===June 1/2010===
JV quantified the amount of DNA in gels run to date using ImageJ software. Results to be posted in working plasmids box.
===June 2/2010===
Objective: Isolate plasmid DNA (BBa_J33204) from DH5α cells
===June 3/2010===
Objective: Ligate pLacI to sRBS-Lum-dT using the three antibiotic assembly method (according to Tom Knight's protocol). Previous ligation had miniscule quantities of DNA in ligation, not surprising it didn't work.
