Team:Washington/Protocols/EnzymeAssayCapD
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(→Enzyme Assay for CapD + CapDcp) |
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=='''Enzyme Assay for CapD + CapDcp'''== | =='''Enzyme Assay for CapD + CapDcp'''== | ||
Run one transpeptidase reaction and one hydrolysis reaction per enzyme | Run one transpeptidase reaction and one hydrolysis reaction per enzyme | ||
+ | |||
'''Prepare Transpeptidase Reactions''' | '''Prepare Transpeptidase Reactions''' | ||
Revision as of 19:56, 14 September 2010
Enzyme Assay for CapD + CapDcp
Run one transpeptidase reaction and one hydrolysis reaction per enzyme
Prepare Transpeptidase Reactions
Prepare Hydrolysis Reactions
Final Concentrations
- Enzyme (CapD) 5nM (0.00025mg/mL)
- Amino Acid (L-Glutamate)0 or 5mM
- Substrate 50nM
- HEPES (7.4pH) 25mM
- 0.1% Tween
- Final Reaction Volume 100uL