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Team:Washington/Protocols/EnzymeAssayCapD
From 2010.igem.org
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<!---------------------------------------PAGE CONTENT GOES BELOW THIS----------------------------------------> | <!---------------------------------------PAGE CONTENT GOES BELOW THIS----------------------------------------> | ||
=='''Enzyme Assay for CapD + CapDcp'''== | =='''Enzyme Assay for CapD + CapDcp'''== | ||
| - | + | Run one transpeptidase reaction and one hydrolysis reaction per enzyme | |
| + | '''Prepare Transpeptidase Reactions''' | ||
| + | |||
| + | |||
| + | '''Prepare Hydrolysis Reactions''' | ||
| + | |||
'''Final Concentrations''' | '''Final Concentrations''' | ||
Revision as of 19:55, 14 September 2010
Enzyme Assay for CapD + CapDcp
Run one transpeptidase reaction and one hydrolysis reaction per enzyme Prepare Transpeptidase Reactions
Prepare Hydrolysis Reactions
Final Concentrations
- Enzyme (CapD) 5nM (0.00025mg/mL)
- Amino Acid (L-Glutamate)0 or 5mM
- Substrate 50nM
- HEPES (7.4pH) 25mM
- 0.1% Tween
- Final Reaction Volume 100uL
