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Team:Washington/Protocols/EnzymeAssayCapD
From 2010.igem.org
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==Enzyme Assay for CapD + CapDcp== | ==Enzyme Assay for CapD + CapDcp== | ||
| + | *Run one transpeptidase reaction and one hydrolysis reaction per enzyme | ||
| + | *=='''Transpeptidation'''== | ||
'''Final Concentrations''' | '''Final Concentrations''' | ||
*Enzyme (CapD) 5nM (0.00025mg/mL) | *Enzyme (CapD) 5nM (0.00025mg/mL) | ||
Revision as of 19:52, 14 September 2010
Enzyme Assay for CapD + CapDcp
- Run one transpeptidase reaction and one hydrolysis reaction per enzyme
- ==Transpeptidation==
Final Concentrations
- Enzyme (CapD) 5nM (0.00025mg/mL)
- Amino Acid (L-Glutamate)0 or 5mM
- Substrate 50nM
- HEPES (7.4pH) 25mM
- 0.1% Tween
- Final Reaction Volume 100uL
