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Team:Washington/Protocols/EnzymeAssayCapD
From 2010.igem.org
(Difference between revisions)
(→Enzyme Assay for CapD + CapDcp) |
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*Amino Acid (L-Glutamate)0 or 5mM | *Amino Acid (L-Glutamate)0 or 5mM | ||
*Substrate 50nM | *Substrate 50nM | ||
| - | * | + | *HEPES (7.4pH) 25mM |
*0.1% Tween | *0.1% Tween | ||
*Final Reaction Volume 100uL | *Final Reaction Volume 100uL | ||
Revision as of 19:50, 14 September 2010
Enzyme Assay for CapD + CapDcp
Final Concentrations
- Enzyme (CapD) 5nM (0.00025mg/mL)
- Amino Acid (L-Glutamate)0 or 5mM
- Substrate 50nM
- HEPES (7.4pH) 25mM
- 0.1% Tween
- Final Reaction Volume 100uL
