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Team:Washington/Protocols/EnzymeAssayCapD
From 2010.igem.org
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| + | ==Enzyme Assay for CapD + CapDcp== | ||
| + | '''Final Concentrations''' | ||
| + | *Enzyme (CapD) 5nM (0.00025mg/mL) | ||
| + | *Amino Acid (L-Glutamate)0 or 5mM | ||
| + | *Substrate 50nM | ||
| + | *Hepes (7.4pH) 25mM | ||
| + | *0.1% Tween | ||
| + | *Final Reaction Volume 100uL | ||
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Revision as of 19:50, 14 September 2010
Enzyme Assay for CapD + CapDcp
Final Concentrations
- Enzyme (CapD) 5nM (0.00025mg/mL)
- Amino Acid (L-Glutamate)0 or 5mM
- Substrate 50nM
- Hepes (7.4pH) 25mM
- 0.1% Tween
- Final Reaction Volume 100uL
