Team:UNIPV-Pavia/Calendar/September/settimana3
From 2010.igem.org
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m (→September, 14th) |
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==September, 14th== | ==September, 14th== | ||
Miniprep and quanfification of: | Miniprep and quanfification of: | ||
- | *I26: ng/ul | + | *I26: 69,7 ng/ul |
- | *I31: ng/ul | + | *I31: 89,5 ng/ul |
- | *I34: ng/ul | + | *I34: 147,6 ng/ul |
3-hours digestion: | 3-hours digestion: | ||
*I26: XbaI-PstI (Insert) | *I26: XbaI-PstI (Insert) | ||
Line 72: | Line 72: | ||
[[Image:UNIPV10_14_09_10_gel_run_cut_phasins_2step_final.jpg|thumb|300px|center| Gel run/cut for digested I26, I31 and I34.]] | [[Image:UNIPV10_14_09_10_gel_run_cut_phasins_2step_final.jpg|thumb|300px|center| Gel run/cut for digested I26, I31 and I34.]] | ||
and gel extraction: | and gel extraction: | ||
- | *I26 (X-P): | + | *I26 (X-P): ng/ul |
- | *I31 (E-S): | + | *I31 (E-S): ng/ul |
- | *I34 (E-X): | + | *I34 (E-X): ng/ul |
We already had digested DNA so we could perform new ON ligations | We already had digested DNA so we could perform new ON ligations |
Revision as of 12:10, 14 September 2010
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