Team:UC Davis/notebook/initiator.html

From 2010.igem.org

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<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/hydration.html" class="help">Hydrated</a> parts: BBa_B0034, BBa_C0051, BBa_B0015, BBa_R0082.</li>
<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/hydration.html" class="help">Hydrated</a> parts: BBa_B0034, BBa_C0051, BBa_B0015, BBa_R0082.</li>
<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/transformation.html" class="help">Transformed</a> these parts into DH5α competent cells.</li>
<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/transformation.html" class="help">Transformed</a> these parts into DH5α competent cells.</li>
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</ul><p><br />
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<a name="rbsc0051"><h1>Ligating RBS to BBa_C0051</h1></a>
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<ul>
<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/culture.html" class="help">Cultured</a> cells with BBa_B0034 and BBa_C0051</li>
<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/culture.html" class="help">Cultured</a> cells with BBa_B0034 and BBa_C0051</li>
<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/miniprep.html" class="help">Miniprepped</a> BBa_B0034 and BBa_C0051</li>
<li><a href="https://2010.igem.org/Team:UC_Davis/protocols/miniprep.html" class="help">Miniprepped</a> BBa_B0034 and BBa_C0051</li>

Revision as of 03:35, 14 September 2010

The Initiator

  • Hydrated parts: BBa_B0034, BBa_C0051, BBa_B0015, BBa_R0082.
  • Transformed these parts into DH5α competent cells.


Ligating RBS to BBa_C0051

Digest with SpeI & Pst
Digest with Xba & Pst

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)