From 2010.igem.org
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| | *I34: E-X (Vector) | | *I34: E-X (Vector) |
| | Gel run/cut of samples | | Gel run/cut of samples |
| - | [[Image:UNIPV10_14_09_10_gel_run_cut_phasins_2step_final.jpg|thumb|200px|center| Gel run/cut for digested I26, I31 and I34.]] | + | [[Image:UNIPV10_14_09_10_gel_run_cut_phasins_2step_final.jpg|thumb|300px|center| Gel run/cut for digested I26, I31 and I34.]] |
| | and gel extraction: | | and gel extraction: |
| | *I26 (X-P): ng/ul | | *I26 (X-P): ng/ul |
Revision as of 19:39, 13 September 2010
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SEPTEMBER: WEEK 3
September, 13th
Unfortunately we discovered we tried to ligate I32 (E-S) to I37 (X-P) instead of I37 (E-X) ;-(
So this step will be repeated.
Trasformation of ligations
into E. coli DH5-alpha.
Inoculum of
into 5 ml LB+Amp for ligations of the following day.
September, 14th
Miniprep and quanfification of:
- I26: ng/ul
- I31: ng/ul
- I34: ng/ul
3-hours digestion:
- I26: XbaI-PstI (Insert)
- I31: EcoRI-SpeI (Insert)
- I34: E-X (Vector)
Gel run/cut of samples
 Gel run/cut for digested I26, I31 and I34. and gel extraction:
- I26 (X-P): ng/ul
- I31 (E-S): ng/ul
- I34 (E-X): ng/ul
We already had digested DNA so we could perform new ON ligations
- IXX: I32 (E-S) + I37 (E-X)
- IXX: I21 (E-S) + I34 (E-X)
- IXX: I31 (E-S) + I37 (E-X)
and repeat
- I53: I32 (E-S) + I37 (E-X)
September, 15th
September, 16th
September, 17th
September, 18th
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