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Team:UNIPV-Pavia/Calendar/September/settimana3
From 2010.igem.org
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==September, 14th== | ==September, 14th== | ||
| + | Miniprep and quanfification of: | ||
| + | *I26: ng/ul | ||
| + | *I31: ng/ul | ||
| + | *I34: ng/ul | ||
| + | 3-hours digestion: | ||
| + | *I26: XbaI-PstI | ||
| + | *I31: EcoRI-SpeI | ||
| + | *I34: E-X | ||
| + | Gel run/cut of samples | ||
| + | [[Image:UNIPV10_14_09_10_gel_run_cut_phasins_2setp_final.jpg|thumb|200px|center| Gel run/cut for I26, I31 and I34.]] | ||
| + | and gel extraction: | ||
| + | *I26 (X-P): ng/ul | ||
| + | *I31 (E-S): ng/ul | ||
| + | *I34 (E-X): ng/ul | ||
| + | |||
| + | We already had digested DNA so we could perform new ON ligations | ||
| + | *IXX: I32 (E-S) + I37 (E-X) | ||
| + | *IXX: I21 (E-S) + I34 (E-X) | ||
| + | *IXX: I31 (E-S) + I37 (E-X) | ||
| + | and repeat | ||
| + | *I53: I32 (E-S) + I37 (E-X) | ||
<div align="right"><small>[[#indice|^top]]</small></div> | <div align="right"><small>[[#indice|^top]]</small></div> | ||
Revision as of 19:37, 13 September 2010
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