"
Team:TU Delft/Modeling/MFA/NO3
From 2010.igem.org
(Difference between revisions)
Lbergwerff (Talk | contribs) (→NO3 as electron acceptor) |
Lbergwerff (Talk | contribs) (→NO3 as electron acceptor) |
||
| Line 2: | Line 2: | ||
In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced. | In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced. | ||
| + | |||
In this scenario the oxidative phosphorylation was disabled and a replacement was implemented; | In this scenario the oxidative phosphorylation was disabled and a replacement was implemented; | ||
NO3- + NADH + 8 H+ -> NO2- + NAD+ + H+ + 6 Hex+ + H2O | NO3- + NADH + 8 H+ -> NO2- + NAD+ + H+ + 6 Hex+ + H2O | ||
| + | |||
NO3 import | NO3 import | ||
| + | |||
NO2 export | NO2 export | ||
| + | |||
This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP. | This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP. | ||
Revision as of 09:06, 13 September 2010
NO3 as electron acceptor
In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced.
In this scenario the oxidative phosphorylation was disabled and a replacement was implemented;
NO3- + NADH + 8 H+ -> NO2- + NAD+ + H+ + 6 Hex+ + H2O
NO3 import
NO2 export
This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP.
The goal of implementing this pathway is to reduce the oxygen requirement of E. coli.
