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Team:TU Delft/Modeling/MFA/NO3
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In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced. | In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced. | ||
Revision as of 09:06, 13 September 2010
NO3 as electron acceptor
In oily environments oxygen diffuses more difficult into the water phase. The oxygen is used for the oxidative phosphorylation, regenerating NADH, and for the first step in the hydrocarbon degradation. To be more efficient with oxygen an additional electron acceptor was introduced.
In this scenario the oxidative phosphorylation was disabled and a replacement was implemented;
NO3- + NADH + 8 H+ -> NO2- + NAD+ + H+ + 6 Hex+ + H2O
This reaction uses NO3 as an electron acceptor to regenerate NADH and export protons to generate ATP.
The goal of implementing this pathway is to reduce the oxygen requirement of E. coli.
