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Team:Newcastle/10 September 2010
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| + | =''yne''A= | ||
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| + | Nanodrop results: | ||
| + | |||
| + | #158.7 | ||
| + | #261.2 | ||
| + | #262.9 | ||
| + | #204.7 | ||
| + | #262.9 | ||
| + | #202.1 | ||
| + | #174.8 | ||
| + | #226.6 | ||
| + | #229.1 | ||
| + | #164.5 | ||
| + | #177.8 | ||
| + | #225.1 | ||
| + | #281.5 | ||
| + | #249.3 | ||
| + | #192.2 | ||
| + | #239.3 | ||
| + | #195.1 | ||
=Arabinose-inducible promoter characterisation= | =Arabinose-inducible promoter characterisation= | ||
Revision as of 13:39, 10 September 2010
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Contents |
yneA
Nanodrop results:
- 158.7
- 261.2
- 262.9
- 204.7
- 262.9
- 202.1
- 174.8
- 226.6
- 229.1
- 164.5
- 177.8
- 225.1
- 281.5
- 249.3
- 192.2
- 239.3
- 195.1
Arabinose-inducible promoter characterisation
...
Miniprep of BBa_I13401 (GFP coding sequence + terminator)
..from last night's overnight cultures from the 8th of september transformation.
Double digests
.. double digests for standard BioBrick assembly.. cut the arabinose-inducible promoter BioBrick with EcoRI and SpeI, cut gfp+terminator BioBrick with EcoRI and XbaI, for later ligation..
Gel extraction of digests
Ligation
   
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