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Team:Newcastle/6 September 2010
From 2010.igem.org
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== Methods == | == Methods == | ||
| - | The [[Team:Newcastle/Growing_an_overnight_cultures| Growing Overnight Cultures]] protocol was followed. The colonies were grown on LB with chloramphenicol. Altogether 16 colonies were produced on the plates and were left overnight. | + | The [[Team:Newcastle/Growing_an_overnight_cultures| Growing Overnight Cultures]] protocol was followed. The colonies were grown on LB with chloramphenicol. Altogether 16 colonies were produced on the plates and were left overnight at 37°C. |
=Sucrose/Levan's glue= | =Sucrose/Levan's glue= | ||
Revision as of 08:46, 8 September 2010
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Contents |
rocF
Aims
rocF miniprep: we aim to purify rocF in p.....
Materials and protocol
Results
Nanodrop
rocF
Concentration of DNA in ng/µl
- 93.1
- 102.0
- 99.4
- 101.0
- 98.8
- 104.7
- 93.1
- 57.9
- 81.9
Subtilin Immunity BioBrick
Aims
Overnight cultures of all the colonies from the 'SI 1/9/10' plates was carried out today.
Methods
The Growing Overnight Cultures protocol was followed. The colonies were grown on LB with chloramphenicol. Altogether 16 colonies were produced on the plates and were left overnight at 37°C.
Sucrose/Levan's glue
The sucrose cultures and controls were compared. The cultures grown on sucrose should be considerably thicker. The overnight cultures of the different strains were also grown on solid agar plates with and without sucrose.
PHOTOS
   
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