Team:UC Davis/protocols/agarplates.html

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<p class="header"><b>Nutrient Agar Plates</b></a><p class="small">By David Larsen</a><p>
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<p class="header"><b>Nutrient Agar Plates</b></a><p>
<a name="materials"><h1>Materials</h1></a>
<a name="materials"><h1>Materials</h1></a>
               <p> You will need: <br />
               <p> You will need: <br />
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<li>Autoclave. </li>
<li>Autoclave. </li>
<li>Let it cool, but not cool enough to let it solidify. It is recommended to keep it in 45 degree waterbath. </li>
<li>Let it cool, but not cool enough to let it solidify. It is recommended to keep it in 45 degree waterbath. </li>
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<li>Add the appropriate amount of antibiotic of your choice.  Recommended amounts are posted on openwetware.  Allow it mix properly.</li>
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<li>Add the appropriate amount of antibiotic of your choice.  Recommended amounts are posted on <a href="http://www.openwetware.org/wiki/Main_Page" class="help">openwetware.org</a>.  Mix antibiotic.</li>
<li>Pour into sterilized plates. </li>
<li>Pour into sterilized plates. </li>
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<p>
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<a name="purpose"><h1>Purpose</h1></a>
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Nutrient agar plates are used to grow and select for desired microbes. 
      
      
<a name="references"><h1>References</h1></a>
<a name="references"><h1>References</h1></a>
<ul>
<ul>
<li>Written by David Larsen 9/11/08 </li>
<li>Written by David Larsen 9/11/08 </li>
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<li>From Cold Spring Harbor protocol </li>
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<li>Cold Spring Harbor protocol </li>
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<li><a href="#materials" class="help">Materials</a></li>
<li><a href="#materials" class="help">Materials</a></li>
<li><a href="#procedure" class="help">Procedure</a></li>
<li><a href="#procedure" class="help">Procedure</a></li>
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<li><a href="#purpose" class="help">Purpose</a></li>
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<li><a href="#references" class="help">References</a></li>
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Latest revision as of 23:08, 7 September 2010

Nutrient Agar Plates

Materials

You will need:

  • Deionized water
  • Tryptone
  • NaCl
  • Yeast Extract
  • Agar

Procedure

  • Combine 10g tryptone, 5g yeast extract, 10g NaCl, 15g agar, and 950 mL of deionized water into a large beaker/Erlenmeyer flask.
  • Mix until dissolved using a stir bar.
  • Check to see if the pH is 7.0 using a pH meter. If needed, add 5N NaOH to adjust the pH to 7.0.
  • Fill solution to 1L with deionized water.
  • Autoclave.
  • Let it cool, but not cool enough to let it solidify. It is recommended to keep it in 45 degree waterbath.
  • Add the appropriate amount of antibiotic of your choice. Recommended amounts are posted on openwetware.org. Mix antibiotic.
  • Pour into sterilized plates.

Purpose

Nutrient agar plates are used to grow and select for desired microbes.

References
  • Written by David Larsen 9/11/08
  • Cold Spring Harbor protocol

We would like to take a moment to thank all of our sponsors for their very generous donations, as we could not have done this without your help!

We would also like to thank and acknowledge:
Our Advisors
Marc Facciotti
Ilias Tagkopoulos
Technical Guidance
David Larsen
Andrew Yao
Visiting iGEMer
Jia Li of Zhejiang University (TEAM ZJU-China)
cI Promoter Screen
Drew Endy - Stanford
Thomas Schneider - NIH
Want to sponsor us? Send an email to mtfacciotti@ucdavis.edu to discuss various ways you can help! :)

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